Department of Ophthalmology, University Medicine, Greifswald, Germany.
Med Sci Monit. 2012 May;18(5):BR188-92. doi: 10.12659/msm.882728.
A large number of artificial tears is widely used to treat dry eye symptoms. To test the efficacy of these drugs independent of individual parameters in vitro models are required. As described previously, we employed a reproducible in vitro cell culture system to evaluate the desiccation protection capability of some artificial tears. In THE PRESENT PAPER DATA IS PRESENTED OF ANOTHER SET OF PHARMACEUTICAL AGENTS.
MATERIAL/METHODS: Conjunctival epithelial cell line Chang 1-5c-4 (series 1) and the corneal cell line 2.040 pRSV-T (series 2) were cultured under standard conditions. Confluent cells were wetted for 20 min with artificial tears (Arufil Uno, Arufil, Lacrimal, Lacophthal sine, Siccaprotect, Tears Again, Vidisept EDO, Vistil, Wet Comod) or PBS as a control. After exposure to a constant air flow for 0, 15, 30 and 45 minutes respectively, cells were incubated with the vital dye alamarBlue. Subsequently, absorption of the oxidised form of the dye was assessed using an ELISA-Reader.
Cell best survival rates in series 1 after 15 min were found for Lacrimal (0.89), Wet Comod (0.84) compared to PBS (0.66) and in series 2 for Vidisept EDO (0.57) and Lacrimal (0.56) compared to PBS (0.01). After 45 min highest survival was seen in series 1 for Lacrimal (0.46) and Lacophthal sine (0.36) compared to PBS (0.33) and in series 2 for Lacrimal (-0.06) and Arufil (-0.16) compared to PBS (-0.23).
Both cell lines tested showed different susceptibility towards desiccation and the artificial tears showed differences in preventing cells from desiccation.
大量的人工泪液被广泛用于治疗干眼症症状。为了独立于个体参数测试这些药物的疗效，需要体外模型。如前所述，我们采用可重复的体外细胞培养系统来评估一些人工泪液的干燥保护能力。在本研究中，我们介绍了另一组药物的实验数据。
材料/方法：使用标准条件培养结膜上皮细胞系 Chang 1-5c-4（系列 1）和角膜细胞系 2.040 pRSV-T（系列 2）。将细胞铺满培养瓶后，用人工泪液（Arufil Uno、Arufil、Lacrimal、Lacophthal sine、Siccaprotect、Tears Again、Vidisept EDO、Vistil、Wet Comod）或 PBS 作为对照，分别润湿 20 分钟。然后，使细胞暴露在恒定气流中 0、15、30 和 45 分钟后，用活细胞染料 alamarBlue 孵育细胞。随后，使用 ELISA 读数仪检测氧化型染料的吸收情况。
在系列 1 中，15 分钟后，Lacrimal（0.89）和 Wet Comod（0.84）的细胞存活率最佳，而 PBS 为 0.66；在系列 2 中，Vidisept EDO（0.57）和 Lacrimal（0.56）的细胞存活率最佳，而 PBS 为 0.01。在系列 1 中，45 分钟后，Lacrimal（0.46）和 Lacophthal sine（0.36）的细胞存活率最高，而 PBS 为 0.33；在系列 2 中，Lacrimal（-0.06）和 Arufil（-0.16）的细胞存活率最高，而 PBS 为-0.23。
两种测试的细胞系对干燥均有不同的敏感性，且人工泪液在防止细胞干燥方面也存在差异。
