通过逆转录-PCR 结合寡核苷酸阵列杂交技术快速特异性检测拉萨病毒。
Rapid and specific detection of Lassa virus by reverse transcription-PCR coupled with oligonucleotide array hybridization.
机构信息
Department of Virology, Bernhard-Nocht-Institute for Tropical Medicine, Hamburg, Germany.
出版信息
J Clin Microbiol. 2012 Jul;50(7):2496-9. doi: 10.1128/JCM.00998-12. Epub 2012 Apr 25.
Abstract
To facilitate sequence-specific detection of DNA amplified in a diagnostic reverse transcription (RT)-PCR for Lassa virus, we developed an array featuring 47 oligonucleotide probes for post-PCR hybridization of the amplicons. The array procedure may be performed with low-tech equipment and does not take longer than agarose gel detection.
摘要
为了便于在拉沙病毒诊断逆转录（RT）-PCR 中扩增的 DNA 的序列特异性检测，我们开发了一种阵列，该阵列具有 47 个寡核苷酸探针，用于扩增子的 PCR 后杂交。该阵列程序可以使用低技术设备进行，并且不会比琼脂糖凝胶检测花费更长时间。
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Am J Trop Med Hyg. 1993 Aug;49(2):214-21. doi: 10.4269/ajtmh.1993.49.214.
5
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J Clin Microbiol. 1994 Dec;32(12):2898-903. doi: 10.1128/jcm.32.12.2898-2903.1994.
6
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J Clin Microbiol. 1990 Dec;28(12):2689-92. doi: 10.1128/jcm.28.12.2689-2692.1990.
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