Lunkenheimer K, Hufert F T, Schmitz H
Department of Virology, Bernhard-Nocht Institute for Tropical Medicine, Hamburg, Federal Republic of Germany.
J Clin Microbiol. 1990 Dec;28(12):2689-92. doi: 10.1128/jcm.28.12.2689-2692.1990.
Suitable oligonucleotide primers and probes were synthesized to amplify Lassa virus (Josiah strain)-specific nucleoprotein and glycoprotein gene fragments by using reverse transcription combined with the polymerase chain reaction (PCR). Our primers did not amplify the related lymphocytic choriomeningitis virus. By using PCR, about 50 50% tissue culture infective doses could be detected in the supernatant of infected cells. Furthermore, in all five serum specimens and four of five urine specimens of patients with acute Lassa fever, viral RNA could be demonstrated. Negative results were obtained with all serum and urine specimens of healthy subjects. Our data suggest that PCR may be applied as an alternative to virus isolation in the rapid diagnosis of Lassa fever.
合成了合适的寡核苷酸引物和探针，通过逆转录结合聚合酶链反应（PCR）扩增拉沙病毒（约西亚株）特异性核蛋白和糖蛋白基因片段。我们的引物未能扩增相关的淋巴细胞性脉络丛脑膜炎病毒。通过PCR，可在感染细胞的上清液中检测到约50个50%组织培养感染剂量。此外，在急性拉沙热患者的所有5份血清标本和5份尿液标本中的4份中，均可检测到病毒RNA。健康受试者的所有血清和尿液标本检测结果均为阴性。我们的数据表明，PCR可作为病毒分离的替代方法用于拉沙热的快速诊断。
