Wei Yi-Liang, Li Cai-Xia, Jia Jing, Hu Lan, Liu Yao
Key Laboratory of Ministry of Public Health for Forensic Science, Department of Forensic Science, School of Medicine, Xi'an Jiaotong University, Xi'an 710061, China.
J Forensic Sci. 2012 Nov;57(6):1448-56. doi: 10.1111/j.1556-4029.2012.02154.x. Epub 2012 Apr 26.
As a powerful alternative to short tandem repeat (STR) profiling, we have developed a novel panel of 47 single nucleotide polymorphisms (SNPs) for DNA profiling and ABO genotyping. We selected 42 of the 47 SNPs from a panel of 86 markers that were previously validated as universal individual identification markers and identified five additional SNPs including one gender marker and four ABO loci. Match probability of the 42 validated SNPs was found to be 9.5 × 10(-18) in Han Chinese. SNP analysis correctly assessed a panel of historical cases, including both paternity identifications in trios and individual identifications. In addition, while STR profiling of degraded DNA provided information for 11 loci of 16 potential markers with low peak intensities, SNPstream(®) genotyping was sufficient to identify all 47 SNPs. In summary, SNP analysis is equally effective as STR profiling, but appears more suited for individual identification than STR profiling in cases where DNA may be degraded.
作为短串联重复序列（STR）分析的一种强大替代方法，我们开发了一组用于DNA分析和ABO基因分型的47个单核苷酸多态性（SNP）的新型面板。我们从一组86个标记中选择了47个SNP中的42个，这些标记先前已被验证为通用个体识别标记，并另外鉴定了5个SNP，包括1个性别标记和4个ABO基因座。在汉族人群中，42个经过验证的SNP的匹配概率为9.5×10（-18）。SNP分析正确评估了一组历史案例，包括三联体中的亲子鉴定和个体识别。此外，虽然对降解DNA的STR分析为16个潜在标记中的11个位点提供了低峰强度信息，但SNPstream（®）基因分型足以识别所有47个SNP。总之，SNP分析与STR分析同样有效，但在DNA可能降解的情况下，似乎比STR分析更适合个体识别。
