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一种新技术对抗体特异性的证明。凝胶电泳衍生的酶联免疫吸附测定法(GEDELISA)及其在猫白血病病毒特异性抗体中的应用。

The demonstration of antibody specificity by a new technique. The gel electrophoresis-derived enzyme-linked immunosorbent assay (GEDELISA) and its application to antibodies specific for feline leukemia virus.

作者信息

Lutz H, Higgins J, Pedersen N C, Theilen G H

出版信息

J Histochem Cytochem. 1979 Aug;27(8):1216-8. doi: 10.1177/27.8.225379.

DOI:10.1177/27.8.225379
PMID:225379
Abstract

By combining the high resolution of sodium dodecylsulfate polyacrylamide gel electrophoresis with the sensitivity of enzyme-linked immunosorbent assay (ELISA) antibodies specific for different feline leukemia virus components are characterized. Based on the same principle, Concanavalin A binding sites of FeLV components are also detected.

摘要

通过将十二烷基硫酸钠聚丙烯酰胺凝胶电泳的高分辨率与酶联免疫吸附测定(ELISA)的灵敏度相结合,对不同猫白血病病毒成分具有特异性的抗体得以表征。基于相同原理,还检测了猫白血病病毒成分的伴刀豆球蛋白A结合位点。

相似文献

1
The demonstration of antibody specificity by a new technique. The gel electrophoresis-derived enzyme-linked immunosorbent assay (GEDELISA) and its application to antibodies specific for feline leukemia virus.一种新技术对抗体特异性的证明。凝胶电泳衍生的酶联免疫吸附测定法(GEDELISA)及其在猫白血病病毒特异性抗体中的应用。
J Histochem Cytochem. 1979 Aug;27(8):1216-8. doi: 10.1177/27.8.225379.
2
Humoral immune reactivity to feline leukemia virus and associated antigens in cats naturally infected with feline leukemia virus.自然感染猫白血病病毒的猫对猫白血病病毒及相关抗原的体液免疫反应性。
Cancer Res. 1980 Oct;40(10):3642-51.
3
Course of feline leukemia virus infection and its detection by enzyme-linked immunosorbent assay and monoclonal antibodies.猫白血病病毒感染的病程及其通过酶联免疫吸附测定和单克隆抗体进行的检测
Am J Vet Res. 1983 Nov;44(11):2054-9.
4
Induction of protective immune response in cats by vaccination with feline leukemia virus iscom.通过接种猫白血病病毒iscom在猫中诱导保护性免疫反应。 (注:“iscom”可能是特定的术语或缩写,这里直接保留英文未翻译,需根据具体背景确定准确含义)
J Immunol. 1985 Jul;135(1):591-6.
5
Enzyme-linked immunosorbent assay methods for detection of feline leukemia virus and feline immunodeficiency virus.用于检测猫白血病病毒和猫免疫缺陷病毒的酶联免疫吸附测定方法。
J Am Vet Med Assoc. 1991 Nov 15;199(10):1336-9.
6
Characterization of monoclonal antibodies directed against the envelope proteins of feline leukemia virus.针对猫白血病病毒包膜蛋白的单克隆抗体的特性分析
Cancer Res. 1984 Aug;44(8):3512-7.
7
[Infection with feline leukemia virus: immunology and serodiagnosis as the basis for control. 1].
Schweiz Arch Tierheilkd. 1984 Jan;126(1):1-22.
8
Evaluation of humoral immunity tests in FeLV infected cats.
Dev Biol Stand. 1990;72:197-200.
9
Influence of antibody infusion on pathogenesis of experimental feline leukemia virus infection.
J Natl Cancer Inst. 1985 Apr;74(4):821-7.
10
Diagnosis of feline leukemia virus infections.
Mod Vet Pract. 1981 Aug;62(8):611-4.

引用本文的文献

1
Detection of xanthine oxidase and immunologically related proteins in fractions from bovine mammary tissue and milk after electrophoresis in polyacrylamide gels containing sodium dodecyl sulphate.在含有十二烷基硫酸钠的聚丙烯酰胺凝胶中进行电泳后,检测来自牛乳腺组织和乳汁的各组分中的黄嘌呤氧化酶及免疫相关蛋白。
Biochem J. 1982 Feb 15;202(2):317-23. doi: 10.1042/bj2020317.
2
Purification of xanthine oxidase from the fat-globule membrane of bovine milk by electrofocusing.通过电聚焦从牛乳脂肪球膜中纯化黄嘌呤氧化酶。
Mol Cell Biochem. 1982 Apr 16;44(1):13-22. doi: 10.1007/BF00573841.
3
Cross-reactivity of Haemophilus somnus antibody in agglutination and complement fixation tests and in the enzyme-linked immunosorbent assay.
睡眠嗜血杆菌抗体在凝集试验、补体结合试验及酶联免疫吸附测定中的交叉反应性。
J Clin Microbiol. 1983 Mar;17(3):500-6. doi: 10.1128/jcm.17.3.500-506.1983.
4
Analysis of antibodies in local and disseminated Neisseria gonorrhoeae infections by means of gel electrophoresis-derived ELISA.通过凝胶电泳衍生的酶联免疫吸附测定法分析局部和播散性淋病奈瑟菌感染中的抗体。
Immunology. 1982 Oct;47(2):283-8.