Biotechnology Research Division, National Fisheries Research and Development Institute, 408-1 Sirang-ri, Gijang-eup, Gijang-Gun, Busan 619-902, Republic of Korea.
Fish Shellfish Immunol. 2012 Jul;33(1):92-8. doi: 10.1016/j.fsi.2012.04.006. Epub 2012 Apr 19.
An approximately 21 kDa antimicrobial protein was purified from an acidified testis extract of olive flounder, Paralichthys olivaceus, by ion-exchange and C(18) reversed-phase HPLC. A comparison of the N-terminal amino acid sequence with those of other known antimicrobial polypeptides revealed high homology between this antimicrobial protein and other histone H1 molecules; thus, it was designated flounder histone H1-like protein (fH1LP). fH1LP showed potent antimicrobial activity against Gram-positive bacteria, including Bacillus subtilis, Staphylococcus aureus, and Streptococcus iniae (minimal effective concentrations [MECs], 2.8-30.0 μg/ml), Gram-negative bacteria, including Aeromonas hydrophila, Escherichia coli D31, Vibrio parahaemolyticus (MECs, 1.4-12.0 μg/ml), and Candida albicans (MEC, 2.0 μg/ml). cDNA cloning and tissue distribution studies of fH1LP indicated that it is constitutively expressed in testis and ovary. The fH1LP expression level was significantly dependent on developmental stage, and decreased dramatically after hatching. However, lipopolysaccharide stimulation did not induce fH1LP mRNA in other immune organs, including the kidney and spleen. These results suggest that fH1LP plays an important role in innate immunity in fish during reproduction, including mating, fertilization, and hatching.
从橄榄石斑鱼（Paralichthys olivaceus）酸性睾丸提取物中通过离子交换和 C(18)反相 HPLC 纯化出一种约 21 kDa 的抗菌蛋白。将该抗菌蛋白的 N 端氨基酸序列与其他已知抗菌多肽进行比较，发现其与其他组蛋白 H1 分子具有高度同源性；因此，将其命名为石斑鱼组蛋白 H1 样蛋白（fH1LP）。fH1LP 对革兰氏阳性菌（包括枯草芽孢杆菌、金黄色葡萄球菌和鳗弧菌）具有很强的抗菌活性（最小有效浓度 [MEC]，2.8-30.0 μg/ml），对革兰氏阴性菌（包括嗜水气单胞菌、大肠杆菌 D31、副溶血弧菌）也具有抗菌活性（MEC，1.4-12.0 μg/ml），对白色念珠菌（MEC，2.0 μg/ml）也具有抗菌活性。fH1LP 的 cDNA 克隆和组织分布研究表明，它在睾丸和卵巢中持续表达。fH1LP 的表达水平与发育阶段显著相关，孵化后急剧下降。然而，脂多糖刺激不会诱导其他免疫器官（包括肾脏和脾脏）中的 fH1LP mRNA 表达。这些结果表明，fH1LP 在鱼类生殖过程中的先天免疫中发挥重要作用，包括交配、受精和孵化。
