College of Food Science and Engineering, Ocean University of China, Qingdao, Shandong 266003, China.
Mar Drugs. 2020 Feb 26;18(3):133. doi: 10.3390/md18030133.
In the present study, histone from immature testis of Atlantic salmon was extracted and identified, and its antibacterial activity after enzymolysis was investigated. Histone extracted from Atlantic salmon () testis using the acid extraction method was successfully identified by LC-MS/MS, and revealed significant inhibitory activity on both the Gram-negative and Gram-positive bacteria. With a low concentration of 10 mg/mL, the observed inhibitory zone diameter (IZD) could significantly reach up to 15.23 mm. After modification of enzymatic hydrolysis by pepsin, histone could be digested to three fragments, while the antibacterial activity increased up to 57.7%. All the results suggested the leftovers from commercial fishing could be utilized for the extraction of antimicrobial peptides.
在本研究中，提取并鉴定了大西洋鲑鱼未成熟睾丸中的组蛋白，并研究了其酶解后的抗菌活性。采用酸提取法从大西洋鲑鱼（）睾丸中提取的组蛋白，经 LC-MS/MS 成功鉴定，并对革兰氏阴性菌和革兰氏阳性菌均表现出显著的抑制活性。在浓度低至 10mg/mL 时，观察到的抑菌圈直径（IZD）可显著达到 15.23mm。经过胃蛋白酶酶解修饰后，组蛋白可被消化为三个片段，同时抗菌活性提高了 57.7%。所有结果表明，商业捕捞的剩余物可用于提取抗菌肽。
