Liu Lubin, Deng Li, Wang Yanzhou, Ge Liangpeng, Chen Yong, Liang Zhiqing
Department of Obstetrics and Gynecology, Southwestern Hospital, Third Military Medical University, Gao-tan Yan Street, Shapingba District, Chongqing 400038, People's Republic of China.
Int Urogynecol J. 2012 Sep;23(9):1271-8. doi: 10.1007/s00192-012-1745-8. Epub 2012 Apr 27.
The present study set out to modify polypropylene vaginal surgical material using porcine urinary bladder matrix (UBM) in order to improve biocompatibility. The aim was to develop a compound scaffold that induced less vaginal erosion and to evaluate host immunoreactivity to this material in vivo.
Forty-eight Sprague-Dawley rats were randomly divided into four equal groups. One group underwent a sham operation, and the other groups underwent vaginal implantation with different materials: UBM (U); UBM + polypropylene (UP); or polypropylene (P). The host tissue response was determined by macro-observation, and by histological and immunohistochemical methods at 7, 14, 21, or 28 days after surgery.
The inflammation reaction was strongest throughout the entire observation time in Group P, but was weaker and had a tendency to decrease with time in Groups U and UP. The presence of the UBM material in the compound scaffold allowed the polypropylene to fuse with newly proliferating surrounding tissue and resulted in less rejection of the material by the host, as indicated by the reduced appearance of CD4-, and CD8-positive cells.
Porcine UBM allowed mechanical isolation of polypropylene, and also reduced the immune reaction to polypropylene. This study suggests that the UBM + polypropylene compound scaffold may be a promising material for clinical use in pelvic reconstruction surgery.
本研究旨在使用猪膀胱基质（UBM）对聚丙烯阴道手术材料进行改性，以提高生物相容性。目的是开发一种引起较少阴道糜烂的复合支架，并在体内评估宿主对该材料的免疫反应。
48只Sprague-Dawley大鼠随机分为四组，每组数量相等。一组进行假手术，其他组分别植入不同材料：UBM（U组）；UBM +聚丙烯（UP组）；或聚丙烯（P组）。通过宏观观察、组织学和免疫组织化学方法在术后7、14、21或28天测定宿主组织反应。
P组在整个观察期内炎症反应最强，而U组和UP组炎症反应较弱且有随时间减弱的趋势。复合支架中UBM材料的存在使聚丙烯与周围新增殖组织融合，宿主对材料的排斥反应减少，表现为CD4和CD8阳性细胞出现减少。
猪UBM可使聚丙烯实现机械隔离，还可降低对聚丙烯的免疫反应。本研究表明，UBM +聚丙烯复合支架可能是盆腔重建手术临床应用中有前景的材料。
