Ge Liangpeng, Liu Lubin, Wei Haoche, Du Lei, Chen Shixuan, Huang Yong, Huang Renshu
Chongqing Academy of Animal Sciences, Chongqing, China Key Laboratory of Pig Industry Sciences, Ministry of Agriculture, Chongqing, China.
Chongqing Health Center for Women and Children, Chongqing Obstetric and Gynecologic Hospital, Chongqing, China.
J Biomater Appl. 2016 Apr;30(9):1385-91. doi: 10.1177/0885328216628469. Epub 2016 Jan 21.
Pelvic organ prolapse (POP) is a serious health issue that affects many adult women. Surgical treatments for POP patients comprise a common strategy in which scaffold materials are used to reconstruct the prolapsed pelvic. However, the existing materials for pelvic reconstruction cannot meet clinical requirements in terms of biocompatibility, mechanics and immunological rejection. To address these concerns, polypropylene (PP) mesh was selected because of its strong mechanical properties. Small intestinal submucosa (SIS) was used to modify the PP mesh via a mussel-inspired polydopamine coating to enhance its biocompatibility. The scanning electron microscopy (SEM) and atomic force microscopy (AFM) results demonstrated that SIS was successfully conjugated on the surface of the PP mesh. Moreover, the cytotoxicity results indicated that the PP mesh and SIS-modified PP mesh were safe to use. Furthermore, in vivo tests demonstrated that the fibroplasia around the implanted site in the SIS-modified PP mesh group was significantly less than the fibroplasia around the PP mesh group. In addition, the immunohistochemistry staining results indicated that the expression of pro-inflammatory macrophages (M1) was substantially lower and that the expression of pro-healing macrophages (M2) was higher in the SIS-modified PP mesh group. Furthermore, ELISA detection indicated that the expression of IL-1β and IL-6 in the SIS-modified PP mesh group was reduced compared with the PP mesh group. These findings suggest that a SIS-modified polypropylene hybrid mesh via a mussel-inspired polydopamine coating is a promising approach in pelvic reconstruction.
盆腔器官脱垂（POP）是一个影响众多成年女性的严重健康问题。针对POP患者的手术治疗是一种常用策略，其中使用支架材料来重建脱垂的盆腔。然而，现有的盆腔重建材料在生物相容性、力学性能和免疫排斥方面无法满足临床需求。为了解决这些问题，选择了聚丙烯（PP）网片，因为其具有较强的力学性能。通过受贻贝启发的聚多巴胺涂层，使用小肠黏膜下层（SIS）对PP网片进行改性，以提高其生物相容性。扫描电子显微镜（SEM）和原子力显微镜（AFM）结果表明，SIS成功地共轭在PP网片表面。此外，细胞毒性结果表明，PP网片和SIS改性PP网片使用安全。此外，体内试验表明，SIS改性PP网片组植入部位周围的纤维增生明显少于PP网片组。此外，免疫组织化学染色结果表明，SIS改性PP网片组促炎巨噬细胞（M1）的表达显著降低，促愈合巨噬细胞（M2）的表达较高。此外，ELISA检测表明，与PP网片组相比，SIS改性PP网片组中IL-1β和IL-6的表达降低。这些发现表明，通过受贻贝启发的聚多巴胺涂层制备的SIS改性聚丙烯混合网片是盆腔重建中一种有前景的方法。
