Department of General Surgery (Colorectal Surgery), Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangdong Institute of Gastroenterology, Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.
Department of Ophthalmology, Biomedical Innovation Center, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510655, China.
Adv Sci (Weinh). 2024 Jun;11(21):e2308590. doi: 10.1002/advs.202308590. Epub 2024 Mar 21.
Pelvic organ prolapse (POP) is one of the most common pelvic floor dysfunction disorders worldwide. The weakening of pelvic connective tissues initiated by excessive collagen degradation is a leading cause of POP. However, the patches currently used in the clinic trigger an unfavorable inflammatory response, which often leads to implantation failure and the inability to simultaneously reverse progressive collagen degradation. Therefore, to overcome the present challenges, a new strategy is applied by introducing puerarin (Pue) into poly(l-lactic acid) (PLLA) using electrospinning technology. PLLA improves the mechanical properties of the patch, while Pue offers intrinsic anti-inflammatory and pro-collagen synthesis effects. The results show that Pue is released from PLLA@Pue in a sustained manner for more than 20 days, with a total release rate exceeding 80%. The PLLA@Pue electrospun patches also show good biocompatibility and low cytotoxicity. The excellent anti-inflammatory and pro-collagen synthesis properties of the PLLA@Pue patch are demonstrated both in vitro in HO-stimulated mouse fibroblasts and in vivo in rat abdominal wall muscle defects. Therefore, it is believed that this multifunctional electrospun patch integrating anti-inflammatory and pro-collagen synthesis properties can overcome the limitations of traditional patches and has great prospects for efficient pelvic floor reconstruction.
盆腔器官脱垂（POP）是全球最常见的盆底功能障碍性疾病之一。由胶原过度降解引发的盆底结缔组织弱化是导致 POP 的主要原因。然而，目前临床上使用的补片会引发不利的炎症反应，这往往导致植入失败和无法同时逆转进行性胶原降解。因此，为了克服当前的挑战，我们采用电纺技术将葛根素（Pue）引入聚（L-乳酸）（PLLA）中，提出了一种新策略。PLLA 改善了补片的机械性能，而 Pue 具有内在的抗炎和促进胶原合成作用。结果表明，Pue 从 PLLA@Pue 中以持续的方式释放超过 20 天，总释放率超过 80%。PLLA@Pue 电纺贴片还表现出良好的生物相容性和低细胞毒性。在 HO 刺激的小鼠成纤维细胞体外和大鼠腹壁肌肉缺损的体内实验中，均证实了 PLLA@Pue 贴片具有优异的抗炎和促进胶原合成特性。因此，人们相信这种具有抗炎和促进胶原合成特性的多功能电纺贴片可以克服传统贴片的局限性，在高效盆底重建方面具有广阔的前景。
