Eisenhauer P, Harwig S S, Szklarek D, Ganz T, Lehrer R I
Department of Medicine, UCLA Center for the Health Sciences 90024.
Infect Immun. 1990 Dec;58(12):3899-902. doi: 10.1128/iai.58.12.3899-3902.1990.
We isolated and characterized a rat neutrophil defensin, RatNP-2, that differs from the previously described defensin RatNP-1 by containing Ser-7 in place of Arg-7. Although the resulting charge difference rendered RatNP-2 easily distinguishable from RatNP-1 on polyacrylamide gel electrophoresis gels, the two defensins exhibited very similar antimicrobial efficacies against Salmonella typhimurium, Staphylococcus aureus, and Candida albicans. The polymorphonuclear leukocytes of Sprague-Dawley rats obtained from one of two breeders also showed a marked polymorphism for defensin RatNP-4. This defensin was absent in two of seven animals and present in 1x or 2x relative amounts in the others. These observations indicate that a striking degree of defensin polymorphism exists in the polymorphonuclear leukocytes of outbred rodents.
我们分离并鉴定了一种大鼠中性粒细胞防御素RatNP-2，它与先前描述的防御素RatNP-1不同，其第7位氨基酸为丝氨酸而非精氨酸。尽管这种电荷差异使得RatNP-2在聚丙烯酰胺凝胶电泳凝胶上很容易与RatNP-1区分开来，但这两种防御素对鼠伤寒沙门氏菌、金黄色葡萄球菌和白色念珠菌表现出非常相似的抗菌效力。从两个饲养者之一获得的斯普拉格-道利大鼠的多形核白细胞中，防御素RatNP-4也表现出明显的多态性。在七只动物中有两只没有这种防御素，其他动物中其相对含量为1倍或2倍。这些观察结果表明，远交啮齿动物的多形核白细胞中存在显著程度的防御素多态性。
