Eisenhauer P B, Harwig S S, Szklarek D, Ganz T, Selsted M E, Lehrer R I
Department of Medicine, University of California, Los Angeles.
Infect Immun. 1989 Jul;57(7):2021-7. doi: 10.1128/iai.57.7.2021-2027.1989.
Three cysteine-rich cationic peptides, designated RatNP-1, RatNP-3, and RatNP-4, were purified from an acid extract of rat polymorphonuclear neutrophils, sequenced, and tested for antimicrobial activity. The peptides ranged from 29 to 32 amino acids in length (Mr, 3,252 to 3,825), and each contained all eight invariantly conserved "framework" residues that are characteristic of defensins. Each of the peptides killed Escherichia coli ML-35, Acinetobacter calcoaceticus HON-1, Staphylococcus aureus 502A, and Candida albicans 820 in vitro. RatNP-1, the most cationic rat defensin, was also the most potent. With this report, a total of 13 distinct defensins have been characterized in the polymorphonuclear leukocytes of four mammalian species. The existence of the defensin system in rats should facilitate investigations of the in vivo role of defensins in experimental infections.
从大鼠多形核中性粒细胞的酸性提取物中纯化出三种富含半胱氨酸的阳离子肽，分别命名为大鼠防御素-1（RatNP-1）、大鼠防御素-3（RatNP-3）和大鼠防御素-4（RatNP-4），对其进行测序并检测抗菌活性。这些肽的长度在29至32个氨基酸之间（分子量为3252至3825），每种肽都含有防御素特有的全部8个恒定保守的“框架”残基。每种肽在体外均可杀死大肠杆菌ML-35、乙酸钙不动杆菌HON-1、金黄色葡萄球菌502A和白色念珠菌820。大鼠防御素-1是大鼠防御素中阳离子性最强的，也是活性最强的。随着本报告的发表，在四种哺乳动物的多形核白细胞中总共鉴定出了13种不同的防御素。大鼠中防御素系统的存在应有助于研究防御素在实验性感染中的体内作用。
