兔巨噬细胞肽MCP-1和MCP-2对小鼠肿瘤细胞的细胞毒性。
Cytotoxicity of rabbit macrophage peptides MCP-1 and MCP-2 for mouse tumor cells.
作者信息
Sheu M J, Baldwin W W, Brunson K W
出版信息
Antimicrob Agents Chemother. 1985 Nov;28(5):626-9. doi: 10.1128/AAC.28.5.626.
Abstract
The cytotoxicity of cationic peptides MCP-1 and MCP-2 isolated from rabbit alveolar macrophages was tested against two tumor cell lines of murine lymphosarcoma origin, RAW117-P and RAW117-H10, and a normal mouse connective tissue fibroblast strain, ATCC CCL1.RAW117-H10 is a highly malignant metastatic variant derived from the less malignant RAW117-P. Our results indicate that these peptides possess a cytotoxic activity against the tumor cells tested but not against the normal cells tested. At concentrations of 30 micrograms/ml, these peptides completely killed RAW117-H10 cells in suspension cultures, while inhibiting growth of RAW117-P for but a limited period of time, up to 48 h, after which growth resumed. RAW117-P cells were killed by concentrations of 40 micrograms/ml. These peptides showed little cytotoxicity for normal mouse connective tissue fibroblasts at concentrations of 40 micrograms/ml.
摘要
对从兔肺泡巨噬细胞中分离出的阳离子肽MCP - 1和MCP - 2，针对两种源自小鼠淋巴肉瘤的肿瘤细胞系RAW117 - P和RAW117 - H10以及一种正常小鼠结缔组织成纤维细胞系ATCC CCL1进行了细胞毒性测试。RAW117 - H10是一种高恶性转移性变体，源自恶性程度较低的RAW117 - P。我们的结果表明，这些肽对所测试的肿瘤细胞具有细胞毒性活性，但对所测试的正常细胞没有。在浓度为30微克/毫升时，这些肽在悬浮培养中完全杀死了RAW117 - H10细胞，而对RAW117 - P的生长仅在有限时间内（长达48小时）有抑制作用，之后生长恢复。RAW117 - P细胞在浓度为40微克/毫升时被杀死。这些肽在浓度为40微克/毫升时对正常小鼠结缔组织成纤维细胞几乎没有细胞毒性。
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