Rahman Mohammad Bozlur, Vandaele Leen, Rijsselaere Tom, Zhandi Mahdi, Maes Dominiek, Shamsuddin Mohammed, Van Soom Ann
Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium.
Reprod Fertil Dev. 2012;24(4):608-18. doi: 10.1071/RD11237.
Exposure of gametes to specific stressors at sublethal levels can enhance the gametes' subsequent performance in processes such as cryopreservation. In the present study, bull spermatozoa were subjected to H₂O₂ for 4 h at 100-, 200- and 500-μM levels; computer-assisted sperm analysis (CASA) and terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling (TUNEL) assay were used for evaluation of subsequent sperm motility and DNA integrity, respectively. Exposure of spermatozoa to H₂O₂ did not affect sperm motility but DNA integrity was negatively affected by 500 μM H₂O₂ compared with mock-exposed spermatozoa, whereas both motility and DNA integrity were affected compared with untreated spermatozoa. Nevertheless, insemination of oocytes with spermatozoa exposed to 200 μM H₂O₂ increased fertilisation, cleavage and blastocyst rates (P < 0.05). Furthermore, the higher blastocyst yield after fertilisation of oocytes with spermatozoa exposed to 200 μM H₂O₂ was related to oocyte diameter, with large-medium oocytes yielding higher blastocyst rates, while small-diameter oocytes consistently failed to develop into blastocysts. In conclusion, the results indicate that exposure of spermatozoa to 200 μM H₂O₂ before sperm-oocyte interaction may enhance in vitro embryo production in cattle. However, this increased embryo production is largely dependent on the intrinsic quality of the oocytes.
将配子暴露于亚致死水平的特定应激源下，可以提高配子在诸如冷冻保存等过程中的后续性能。在本研究中，将公牛精子分别置于100 μM、200 μM和500 μM的过氧化氢中处理4小时；采用计算机辅助精子分析（CASA）和末端脱氧核苷酸转移酶介导的dUTP缺口末端标记（TUNEL）检测法，分别评估后续精子活力和DNA完整性。精子暴露于过氧化氢中对精子活力没有影响，但与模拟暴露的精子相比，500 μM过氧化氢对DNA完整性有负面影响，而与未处理的精子相比，活力和DNA完整性均受到影响。然而，用暴露于200 μM过氧化氢的精子对卵母细胞进行授精，可提高受精率、卵裂率和囊胚率（P < 0.05）。此外，用暴露于200 μM过氧化氢的精子使卵母细胞受精后获得的较高囊胚产量与卵母细胞直径有关，中大型卵母细胞产生的囊胚率较高，而小直径卵母细胞始终无法发育成囊胚。总之，结果表明，在精卵相互作用之前将精子暴露于200 μM过氧化氢可能会提高牛的体外胚胎生产效率。然而，这种胚胎产量的增加在很大程度上取决于卵母细胞的内在质量。
