Morris A G, Burke D C
J Gen Virol. 1979 Apr;43(1):173-81. doi: 10.1099/0022-1317-43-1-173.
NIH 3T3 mouse cells were infected at very high multiplicity with murine sarcoma/leukaemia virus (MSV/MLV) and then cloned. All of the 48 clones obtained were morphologically transformed, all but one showed anchorage-independence of growth, typical of MSV-transformed NIH 3T3 cells and most (91%) produced MSV/MLV. When cells which had been pre-treated with 10(4) units/ml of purified interferon (IF) were infected under the same conditions and then cloned in the presence of the same amount of IF, only 6 of a total of 63 clones were morphologically transformed. All but these 6 showed a degree of anchorage-independence typical of the uninfected parental cells and very few (2.4%) produced virus. Furthermore, the MSV genome could not be rescued in any of the 23 clones tested and only 1 out of 10 clones produced tumours. The properties of these clones remained stable over a period of 10 to 20 passages in the absence of interferon. We conclude that interferon can irreversibly block an early step in the MSV/MLV infectious process.
用鼠肉瘤/白血病病毒（MSV/MLV）以非常高的感染复数感染NIH 3T3小鼠细胞，然后进行克隆。获得的48个克隆全部发生了形态转化，除一个克隆外，其余所有克隆均表现出不依赖贴壁生长的特性，这是MSV转化的NIH 3T3细胞的典型特征，并且大多数（91%）产生MSV/MLV。当用10⁴单位/毫升的纯化干扰素（IF）预处理的细胞在相同条件下感染，然后在相同量的IF存在下进行克隆时，总共63个克隆中只有6个发生了形态转化。除这6个克隆外，其余所有克隆均表现出未感染的亲代细胞典型的一定程度的贴壁依赖性，并且极少（2.4%）产生病毒。此外，在测试的23个克隆中，任何一个克隆都无法拯救出MSV基因组，并且10个克隆中只有1个产生肿瘤。在没有干扰素的情况下，这些克隆的特性在10至20代的时间内保持稳定。我们得出结论，干扰素可以不可逆地阻断MSV/MLV感染过程中的早期步骤。
