Department of Conservative Dentistry, Dental Research Institute, School of Dentistry, Seoul National University, Seoul, Republic of Korea.
Acta Biomater. 2012 Aug;8(8):2986-95. doi: 10.1016/j.actbio.2012.04.032. Epub 2012 Apr 25.
Living dental pulp tissue exposed to the oral environment should be protected with an appropriate pulp capping material to support the dentinogenesis potential of the pulp cells. Mineral trioxide aggregate (MTA) is the material of choice for the treatment of pulp. However, due to cytotoxicity during the initial setting phase of MTA, a new material is required that can act as a barrier to direct contact but facilitate the favorable effect of MTA. This study examined the feasibility of using electrospun poly(ε-caprolactone) fiber (PCL-F) meshes in the MTA-based pulp capping procedures. An experimental pulp capping was performed on the premolars of beagle dogs, and the efficacy of the PCL-F meshes was evaluated after 8 weeks. PCL-F/MTA formed a dentin bridge that was approximately fourfold thicker than that formed by the MTA. Columnar polarized odontoblast-like cells with long processes and tubular dentin-like matrices were observed beneath the dentin bridge in the PCL-F/MTA. The cells were also intensely immunostained for dentin sialoprotein. In cell cultures, PCL-F/MTA reduced cell death to ~8% of that in the MTA group. The proliferation of the cells cultured on PCL-F/MTA was much greater than that of cells cultured on MTA. Furthermore, PCL-F/MTA promoted the differentiation of MDPC23 cells to odontoblast-like cells and biomineralization, as confirmed by the expression of alkaline phosphatase and dentin sialophosphoprotein, and by the deposition of calcium. Based on these histologic findings and the cell responses observed in this study, PCL-F may be used efficiently in the MTA-based dental pulp therapy.
暴露于口腔环境中的活牙髓组织应使用适当的盖髓材料加以保护，以支持牙髓细胞的牙本质生成潜能。三氧化矿物聚合体（MTA）是牙髓治疗的首选材料。然而，由于 MTA 初始凝固阶段的细胞毒性，需要一种新的材料来作为直接接触的屏障，同时促进 MTA 的有利作用。本研究探讨了在 MTA 基盖髓术中使用静电纺聚己内酯纤维（PCL-F）网的可行性。在比格犬的前磨牙上进行了实验性盖髓，8 周后评估了 PCL-F 网的效果。PCL-F/MTA 形成了约为 MTA 形成的牙本质桥的四倍厚的牙本质桥。在 PCL-F/MTA 下方观察到具有长突起的柱状极化成牙本质细胞样细胞和管状牙本质样基质。这些细胞也强烈免疫染色为牙本质涎磷蛋白。在细胞培养中，PCL-F/MTA 将细胞死亡减少到 MTA 组的约 8%。在 PCL-F/MTA 上培养的细胞的增殖远大于在 MTA 上培养的细胞。此外，PCL-F/MTA 促进 MDPC23 细胞向成牙本质细胞样细胞的分化和生物矿化，这通过碱性磷酸酶和牙本质涎磷蛋白的表达以及钙的沉积得到证实。基于这些组织学发现和本研究中观察到的细胞反应，PCL-F 可有效地用于基于 MTA 的牙髓治疗。
