Department of Mechanical Engineering, Colorado State University, Fort Collins, CO, 80523, USA.
J Biomater Sci Polym Ed. 2013;24(13):1529-48. doi: 10.1080/09205063.2013.777228. Epub 2013 Mar 13.
Tissue integration is an important property when inducing transplant tolerance, however, the hemocompatibility of the biomaterial surface also plays an important role in the ultimate success of the implant. Therefore, in order to induce transplant tolerance, it is critical to understand the interaction of blood components with the material surfaces. In this study, we have investigated the adsorption of key blood serum proteins, in vitro adhesion and activation of platelets and clotting kinetics of whole blood on flat polycaprolactone (PCL) surfaces, nanowire (NW) surfaces and nanofiber (NF) surfaces. Previous studies have shown that polymeric nanostructured surfaces improve cell adhesion, proliferation and viability; however it is unclear how these polymeric nanostructured surfaces interact with the blood and its components. Protein adsorption results indicate that while there were no significant differences in total albumin (ALB) adsorption on PCL, NW and NF surfaces, NW surfaces had higher total fibrinogen (FIB) and immunoglobulin-G (IgG) adsorption compared to NF and PCL surfaces. In contrast, NF surfaces had higher surface FIB and IgG adsorption compared to PCL and NW surfaces. Platelet adhesion and viability studies show more adhesion and clustering of platelets on the NF surfaces as compared to PCL and NW surfaces. Platelet activation studies reveal that NW surfaces have the highest percentage of unactivated platelets, whereas NF surfaces have the highest percentage of fully activated platelets. Whole blood clotting results indicate that NW surfaces maintain an increased amount of free hemoglobin during the clotting process compared to PCL and NF surface, indicating less clotting and slower rate of clotting on their surfaces.
组织整合是诱导移植耐受的一个重要特性，然而，生物材料表面的血液相容性在植入物的最终成功中也起着重要作用。因此，为了诱导移植耐受，了解血液成分与材料表面的相互作用至关重要。在这项研究中，我们研究了关键血清蛋白在平整聚己内酯（PCL）表面、纳米线（NW）表面和纳米纤维（NF）表面上的吸附、体外血小板黏附和激活以及全血凝固动力学。先前的研究表明，聚合纳米结构化表面可改善细胞黏附、增殖和活力；然而，尚不清楚这些聚合纳米结构化表面如何与血液及其成分相互作用。蛋白吸附结果表明，尽管 PCL、NW 和 NF 表面上总白蛋白（ALB）的吸附没有显著差异，但 NW 表面上的总纤维蛋白原（FIB）和免疫球蛋白-G（IgG）的吸附高于 NF 和 PCL 表面。相比之下，NF 表面上的 FIB 和 IgG 吸附量高于 PCL 和 NW 表面。血小板黏附和活力研究表明，与 PCL 和 NW 表面相比，NF 表面上的血小板黏附和聚集更多。血小板激活研究表明，NW 表面具有最高百分比的未激活血小板，而 NF 表面具有最高百分比的完全激活血小板。全血凝固结果表明，与 PCL 和 NF 表面相比，NW 表面在凝血过程中保持更多的游离血红蛋白，表明其表面的凝血更少且凝固速度更慢。
