Laboratory of Biology, School of Medicine, University of Patras, 26505 Rio, Patras, Greece.
Bioinformatics. 2012 Jul 1;28(13):1800-1. doi: 10.1093/bioinformatics/bts241. Epub 2012 Apr 27.
We present easyFRAP, a versatile tool that assists quantitative and qualitative analysis of fluorescence recovery after photobleaching (FRAP) data. The user can handle simultaneously large data sets of raw data, visualize fluorescence recovery curves, exclude low quality data, perform data normalization, extract quantitative parameters, perform batch analysis and save the resulting data and figures for further use. Our tool is implemented as a single-screen Graphical User Interface (GUI) and is highly interactive, as it permits parameterization and visual data quality assessment at various points during the analysis.
easyFRAP is free software, available under the General Public License (GPL). Executable and source files, supplementary material and sample data sets can be downloaded at: ccl.med.upatras.gr/easyfrap.html.
我们介绍了 easyFRAP，这是一个通用工具，可协助对荧光漂白后恢复（FRAP）数据进行定量和定性分析。用户可以同时处理大量原始数据数据集，可视化荧光恢复曲线，排除低质量数据，执行数据归一化，提取定量参数，进行批处理分析，并保存生成的数据和图像以供进一步使用。我们的工具实现为单个屏幕的图形用户界面（GUI），具有高度的交互性，因为它允许在分析过程中的各个点进行参数化和可视化数据质量评估。
easyFRAP 是免费软件，根据通用公共许可证（GPL）发布。可执行文件和源文件、补充材料和示例数据集可从以下网址下载：ccl.med.upatras.gr/easyfrap.html。
