Dipartimento di Scienze Chimiche, Università di Padova, via Marzolo 1, I -35131 Padova, Italy.
Chem Commun (Camb). 2012 Jun 7;48(45):5545-59. doi: 10.1039/c2cc30952a. Epub 2012 Apr 30.
The development of synthetic agents able to hydrolytically cleave DNA with high efficiency and selectivity is still a fascinating challenge. Over the years, many examples have been reported reproducing part of the behaviour of the corresponding natural enzymes. Eventually, even the possibility to apply such systems to the manipulation of DNA of higher organisms has been demonstrated. However, efficiency of enzymes is still unrivalled. This feature article discusses the progress reported toward the realization of synthetic nucleases with particular attention to the comprehension of the reaction mechanisms and to the strategies that need to be addressed to obtain more efficient systems.
开发能够高效且选择性地水解 DNA 的合成试剂仍然是一个引人入胜的挑战。多年来，已经有许多报道重现了部分相应天然酶的行为。最终，甚至证明了此类系统可应用于高等生物的 DNA 操作。然而，酶的效率仍然是无与伦比的。本文讨论了在实现合成核酸酶方面的进展，特别关注对反应机制的理解以及需要解决的策略，以获得更有效的系统。
