Bronson R A, Fusi F, Cooper G W, Phillips D M
Division of Reproductive Endocrinology, Health Sciences Center, State University of New York, Stony Brook, 11794-8091.
Hum Reprod. 1990 Aug;5(6):690-6. doi: 10.1093/oxfordjournals.humrep.a137169.
Certain antisperm antibodies (ASAs) in the sera of infertile men or women promote the penetration of zona-free hamster eggs by spermatozoa. We have shown previously that this enhancement of penetration occurs through mechanisms other than an alteration in the acrosomal structure of antibody-labelled spermatozoa. In the present study, small limited populations of antibody-labelled and antibody-free spermatozoa from fertile donors were observed serially by phase contrast, epifluorescence and scanning electron microscopy following their adherence to the oolemma of zona-free hamster eggs. The adherence of ASA-labelled spermatozoa to the zona-free hamster egg was markedly increased and occurred earlier when compared with antibody-free spermatozoa from the same ejaculate. The likelihood of entry of an antibody-labelled spermatozoon into the ooplasm, however, once adherent to the oolemma was not different to that of an antibody-free spermatozoa, and the ultrastructural steps of incorporation of antibody-labelled human spermatozoa were also similar to those observed during fertilization of hamster eggs by spermatozoa of several other species. These observations indicate that ASAs promote polyspermic fertilization of zona-free hamster eggs primarily through their enhancement of sperm--oolemmal binding.
不育男性或女性血清中的某些抗精子抗体（ASA）可促进精子穿透去透明带仓鼠卵。我们之前已经表明，这种穿透增强是通过抗体标记精子顶体结构改变以外的机制发生的。在本研究中，通过相差显微镜、落射荧光显微镜和扫描电子显微镜，对来自可育供体的少量抗体标记精子和未标记精子在附着于去透明带仓鼠卵卵膜后进行连续观察。与来自同一射精样本的未标记精子相比，ASA标记精子与去透明带仓鼠卵的附着明显增加且发生得更早。然而，一旦附着于卵膜，抗体标记精子进入卵质的可能性与未标记精子并无差异，并且抗体标记人类精子纳入的超微结构步骤也与其他几个物种的精子使仓鼠卵受精时观察到的相似。这些观察结果表明，ASA主要通过增强精子与卵膜的结合来促进去透明带仓鼠卵的多精受精。
