在微水反应体系中通过重组大肠杆菌细胞合成手性氰醇。
Synthesis of chiral cyanohydrins by recombinant Escherichia coli cells in a micro-aqueous reaction system.
机构信息
Institut für Molekulare Enzymtechnologie, Heinrich-Heine-Universität Düsseldorf and Forschungszentrum Jülich, Jülich, Germany.
出版信息
Appl Environ Microbiol. 2012 Jul;78(14):5025-7. doi: 10.1128/AEM.00582-12. Epub 2012 Apr 27.
Abstract
Synthesis of chiral cyanohydrins is performed in a monophasic micro-aqueous reaction system using whole recombinant Escherichia coli cells expressing the Arabidopsis thaliana hydroxynitrile lyase (AtHNL). Microscopy studies employing a fusion of AtHNL with a flavin-based fluorescent protein (FbFP) reveal that the cells remain intact in the reaction system.
摘要
在手性氰醇的合成中，使用表达拟南芥羟腈裂解酶（AtHNL）的全重组大肠杆菌细胞在单相微水反应体系中进行。利用 AtHNL 与黄素基荧光蛋白（FbFP）融合的显微镜研究表明，细胞在反应体系中保持完整。
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