de Gonzalo Gonzalo, Lavandera Iván, Faber Kurt, Kroutil Wolfgang
Department of Chemistry, University of Graz, Heinrichstrasse 28, A-8010 Graz, Austria.
Org Lett. 2007 May 24;9(11):2163-6. doi: 10.1021/ol070679c. Epub 2007 May 1.
Mono- and biphasic aqueous-organic solvent systems (50% v v-1) as well as micro-aqueous organic systems (99% v v-1) were successfully employed for the biocatalytic reduction of ketones catalyzed by alcohol dehydrogenase ADH-A from Rhodococcus ruber via hydrogen transfer. A clear correlation between the log P of the organic solvent and the enzyme activity--the higher, the better--was found. The use of organic solvents allowed highly stereoselective enzymatic carbonyl reductions at substrate concentrations close to 2.0 M.
单相和双相水-有机溶剂体系（50% v/v-1）以及微水有机体系（99% v/v-1）已成功用于红球菌ADH-A醇脱氢酶催化的酮类通过氢转移进行的生物催化还原反应。发现有机溶剂的log P与酶活性之间存在明显的相关性——log P越高，酶活性越好。使用有机溶剂可在底物浓度接近2.0 M时实现高度立体选择性的酶促羰基还原反应。
