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建立自交鱼类（大理石无须魮）的发育遗传学。

Establishing developmental genetics in a self-fertilizing fish (Krytolebias marmoratus).

机构信息

Department of Biology, Valdosta State University, Valdosta, GA 31698, USA.

出版信息

Integr Comp Biol. 2012 Dec;52(6):781-91. doi: 10.1093/icb/ics052. Epub 2012 Apr 27.

DOI:10.1093/icb/ics052
PMID:22544288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3501092/
Abstract

Kryptolebias marmoratus is a synchronous hermaphroditic vertebrate that utilizes an ovotestis for reproduction. This fish develops externally, is easy to maintain, and has about a 100-day life cycle, making it a desirable developmental genetic model organism. Here, we present a pilot zygotic mutant screen utilizing the common chemical mutagen, N-ethyl-N-nitrosourea (ENU) to establish genetics in this model species. Selection of clonal stocks and optimal conditions for mutagenizing this fish are presented and the types and frequencies of zygotic mutants are documented in comparison to other fish models. Kryptolebias marmoratus is an exemplar model organism that will complement future developmental genetic screens in vertebrates.

摘要

黄黝鱼是一种同步雌雄同体的脊椎动物，它利用卵睾来繁殖。这种鱼体外发育，易于饲养，生命周期约为 100 天，是一种理想的发育遗传模式生物。在这里，我们利用常见的化学诱变剂 N-乙基-N-亚硝基脲 (ENU) 进行了胚胎期突变体的初步筛选，以在这个模式物种中建立遗传学。本文介绍了克隆株的选择以及诱变这种鱼的最佳条件，并与其他鱼类模型进行了比较，记录了胚胎期突变体的类型和频率。黄黝鱼是一个典范的模式生物，将补充未来脊椎动物的发育遗传筛选。

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TAL effectors: customizable proteins for DNA targeting.TAL 效应因子：可定制的 DNA 靶向蛋白。
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