Institute of Physics, Academia Sinica, Taipei 115, Taiwan.
Biotechnol Adv. 2013 May-Jun;31(3):375-86. doi: 10.1016/j.biotechadv.2012.04.005. Epub 2012 Apr 21.
Our experimental results demonstrate that full-field hard-X-ray microscopy is finally able to investigate the internal structure of cells in tissues. This result was made possible by three main factors: the use of a coherent (synchrotron) source of X-rays, the exploitation of contrast mechanisms based on the real part of the refractive index and the magnification provided by high-resolution Fresnel zone-plate objectives. We specifically obtained high-quality microradiographs of human and mouse cells with 29 nm Rayleigh spatial resolution and verified that tomographic reconstruction could be implemented with a final resolution level suitable for subcellular features. We also demonstrated that a phase retrieval method based on a wave propagation algorithm could yield good subcellular images starting from a series of defocused microradiographs. The concluding discussion compares cellular and subcellular hard-X-ray microradiology with other techniques and evaluates its potential impact on biomedical research.
我们的实验结果表明，全场硬 X 射线显微镜终于能够研究组织内细胞的内部结构。这一结果主要得益于三个因素：使用相干（同步加速器）X 射线源、利用基于折射率实部的对比机制以及高分辨率菲涅尔波带片物镜提供的放大率。我们特别获得了具有 29nm 瑞利空间分辨率的人源和鼠源细胞的高质量微射线照片，并验证了利用适用于亚细胞特征的最终分辨率水平可以实现断层重建。我们还表明，基于波传播算法的相位恢复方法可以从一系列离焦微射线照片中获得良好的亚细胞图像。结论部分将细胞和亚细胞硬 X 射线微射线照相术与其他技术进行了比较，并评估了其对生物医学研究的潜在影响。
