National Synchrotron Radiation Laboratory, University of Science and Technology of China, Hefei, China.
Anal Bioanal Chem. 2012 Sep;404(5):1327-30. doi: 10.1007/s00216-012-6218-x. Epub 2012 Jul 5.
X-ray microscopy based on synchrotron radiation has become a fundamental tool in biology and life sciences to visualize the morphology of a specimen. These studies have particular requirements in terms of radiation damage and the image exposure time, which directly determines the total acquisition speed. To monitor and improve these key parameters, we present a novel X-ray microscopy method using a high-resolution zone plate as the objective and the matching condenser. Numerical simulations based on the scalar wave field theory validate the feasibility of the method and also indicate the performance of X-ray microscopy is optimized most with sub-10-nm-resolution zone plates. The proposed method is compatible with conventional X-ray microscopy techniques, such as computed tomography, and will find wide applications in time-resolved and/or dose-sensitive studies such as living cell imaging.
基于同步辐射的 X 射线显微镜已经成为生物学和生命科学领域中观察样本形态的基本工具。这些研究在辐射损伤和图像曝光时间方面有特殊的要求，这直接决定了总的采集速度。为了监测和改进这些关键参数，我们提出了一种使用高分辨率波带片作为物镜和匹配聚光镜的新型 X 射线显微镜方法。基于标量波场理论的数值模拟验证了该方法的可行性，同时也表明 X 射线显微镜的性能在采用亚 10nm 分辨率波带片时得到了最佳优化。所提出的方法与传统的 X 射线显微镜技术（如计算机层析成像）兼容，并将在时间分辨和/或剂量敏感的研究中得到广泛应用，如活细胞成像。
