Key Laboratory of Resource Biology and Eco-environment of Ministry of Education, Sichuan Key Laboratory of Molecular Biology and Biotechnology, College of Life Science, Sichuan University, Chengdu 610064, People's Republic of China.
Mol Biol Rep. 2012 Aug;39(8):8015-25. doi: 10.1007/s11033-012-1648-2. Epub 2012 May 1.
During post-harvest storage, tuberous roots of sweet potato (Ipomoea batatas L. Lam.) usually undergo a biotic and abiotic stress influencing protein expression pattern and substance contents. This research compared the change of total proteins and carbohydrate content in tuberous roots of sweet potato during the storage period. The result of the two-dimensional electrophoresis analysis demonstrated that there were 25 differentially expressed proteins between day 0 and day 75 during the storage. Among these proteins, 11 proteins were down-regulated and the other 14 were up-regulated. The results from MALDI-TOF-TOF/MS analyses and mascot database searching showed that 11 of the 25 differentially expressed proteins were identified as store-stress regulated proteins. It was also found that the proteins involved in the energy metabolism and the stress-response were drastically up-regulated, whereas those in biomacromolecule synthesis were markedly down-regulated. Meanwhile, under the experimental conditions, the content of the starch and the cellulose was decreased by more than a quarter and the amylase activity was increased moderately.
在采后贮藏过程中，甘薯（Ipomoea batatas L. Lam.）块根通常会经历生物和非生物胁迫，影响蛋白质表达模式和物质含量。本研究比较了甘薯块根在贮藏期间总蛋白质和碳水化合物含量的变化。二维电泳分析的结果表明，在贮藏的第 0 天和第 75 天之间，有 25 种差异表达蛋白。在这些蛋白质中，有 11 种蛋白下调，另外 14 种蛋白上调。MALDI-TOF-TOF/MS 分析和 Mascot 数据库搜索的结果表明，25 种差异表达蛋白中有 11 种被鉴定为贮藏胁迫调节蛋白。研究还发现，参与能量代谢和应激反应的蛋白质显著上调，而参与生物大分子合成的蛋白质则明显下调。同时，在实验条件下，淀粉和纤维素的含量减少了四分之一以上，淀粉酶活性适度增加。
