Imaging Unit - Integrative Oncology Department, British Columbia Cancer Research Centre, Vancouver, BC, Canada.
Photodermatol Photoimmunol Photomed. 2012 Jun;28(3):147-52. doi: 10.1111/j.1600-0781.2012.00660.x.
Skin cancer is the most common type of cancer in humans. Current techniques for identifying normal and neoplastic tissues are either destructive or not sensitive and specific enough. Raman spectroscopy and confocal imaging may obviate many limitations of existing methods by providing noninvasive, high-resolution, and real-time morphological and biochemical analysis of living tissues and cells.
We conducted micro-Raman spectroscopy studies on HaCaT cells, melanocytes (MC) and their malignant counterparts squamous cell carcinoma (SCC) and melanoma (MM) cells, respectively. Reflectance confocal imaging is used as guidance for the spectral measurements.
Significant differences were found between the spectra of HaCaT cells and SCC cells, MC cells and MM cells, as well as all normal cells (HaCaT and MC) and all tumor cells (SCC and MM). Approximately 90% sensitivity and specificity was achieved for all the separations that we performed.
Our results demonstrated the robust capability of confocal Raman spectroscopy in separating different cell lines. The acquired Raman spectra of major types of skin cells and their malignant counterparts will be useful for the interpretation of Raman spectra from in vivo skin. We believe it will eventually help diagnosis of skin cancer and other skin disease in clinical dermatology.
皮肤癌是人类最常见的癌症类型。目前用于识别正常和肿瘤组织的技术要么具有破坏性，要么不够敏感和特异。拉曼光谱和共焦成像技术可以通过对活组织和细胞进行非侵入性、高分辨率和实时的形态和生化分析，避免现有方法的许多局限性。
我们分别对 HaCaT 细胞、黑素细胞（MC）及其恶性 counterparts 鳞状细胞癌（SCC）和黑色素瘤（MM）细胞进行了微拉曼光谱研究。反射共焦成像被用作光谱测量的指导。
在 HaCaT 细胞和 SCC 细胞、MC 细胞和 MM 细胞之间，以及所有正常细胞（HaCaT 和 MC）和所有肿瘤细胞（SCC 和 MM）之间，我们发现了明显的光谱差异。我们进行的所有分离都达到了约 90%的灵敏度和特异性。
我们的结果证明了共焦拉曼光谱在分离不同细胞系方面的强大能力。获得的主要类型皮肤细胞及其恶性对应物的拉曼光谱将有助于解释来自体内皮肤的拉曼光谱。我们相信，它最终将有助于皮肤科临床中皮肤癌和其他皮肤疾病的诊断。
