Departamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de México , Ciudad Universitaria, Mexico City, 04510 DF, Mexico.
J Nat Prod. 2012 May 25;75(5):890-5. doi: 10.1021/np2009412. Epub 2012 May 2.
Commercial preparations of the Mexican herbal drug known as "miracle tea" (Packera candidissima and P. bellidifolia) have been profiled qualitatively by HPLC and GC-MS. Eremophilanes (3-7) were the major components found in the hexane-soluble fraction, while pyrrolizidine alkaloids (PAs) were identified in the alkaloid extracts. The content of free PAs and their N-oxides was determined for a total of 22 samples, and the results showed that the amount of these hepatotoxic compounds (0.0005-0.94% free PAs; 0.0004-0.55% N-oxides), through the presence of retrorsine (1) and senesionine (2) as the main constituents, may reach toxic levels. Hexane-soluble extracts from commercial presentations (dried whole plants) of both species afforded neoadenostylone (3), 6-(2-methylbutanoyloxy)-9-oxo-1-(10)-furanoeremophilene (4), and epineoadenostylone (5), in addition to methyl-4-hydroxyphenylacetate (8) and methyl-2-(1-hydroxy-4-oxocyclohexyl)acetate (9). Also, epicacalone (6) and the new compound 2β-hydroxyneoadenostylone (7) were isolated from P. bellidifolia.
市售的墨西哥草药“奇迹茶”（假泽兰和泽兰）的商业制剂已通过 HPLC 和 GC-MS 进行了定性分析。在正己烷可溶部分中发现了主要成分埃雷莫芬烷（3-7），而生物碱提取物中鉴定出吡咯里西啶生物碱（PAs）。对总共 22 个样品的游离 PAs 及其 N-氧化物含量进行了测定，结果表明，这些肝毒性化合物的含量（0.0005-0.94%游离 PAs；0.0004-0.55% N-氧化物）可能达到有毒水平，其中 retrorsine（1）和 senesionine（2）作为主要成分。商业展示（干整株植物）的正己烷可溶提取物中除了甲基-4-羟基苯乙酸酯（8）和甲基-2-（1-羟基-4-氧环己基）乙酸酯（9）之外，还提供了新腺甾酮（3）、6-（2-甲基丁酰氧基）-9-氧-1-（10）-呋喃埃雷莫芬（4）和表新腺甾酮（5）。此外，还从泽兰中分离出了 epicacalone（6）和新化合物 2β-羟基新腺甾酮（7）。
