Centre for Radiation Protection Research (CRPR), Department of Genetics, Microbiology and Toxicology, Stockholm University, SE-106 91, Stockholm, Sweden.
Nutr J. 2012 May 2;11:29. doi: 10.1186/1475-2891-11-29.
DNA is constantly exposed to reactive oxygen species (ROS), spontaneously arising during the normal oxygen metabolism. ROS may result in temporary as well as permanent modifications in various cellular components such as lipids, proteins and DNA, which may have deleterious consequences. Demonstrating that a dietary supplementation of antioxidants can reduce oxidative DNA damage may provide evidence for the value of such supplementation in prevention of cancer and age related diseases.
The present study was conducted to address whether tomato juice protects against ROS induced by extensive physical exercise in untrained individuals. As a marker of oxidative stress, serum levels of 8-oxodG were monitored using a modified ELISA. An intervention was performed involving 15 untrained healthy subjects who performed a 20 min physical exercise at 80% of maximum pulse using an ergometer bicycle. Blood samples were taken before and one hour after the exercise. The procedure was repeated after 5 weeks with a daily intake of 150 ml tomato juice and followed by a 5 weeks wash-out period and another 5 weeks with a daily intake of tomato juice. The results indicated that a daily intake of tomato juice, equal to 15 mg lycopene per day, for 5 weeks significantly reduced the serum levels of 8-oxodG after an extensive physical exercise.
These data strongly suggest that tomato juice has a potential antioxidant effect and may reduce the elevated level of ROS induced by oxidative stress.
DNA 不断受到活性氧（ROS）的影响，ROS 在正常的氧气代谢过程中自发产生。ROS 可能导致脂质、蛋白质和 DNA 等各种细胞成分的暂时和永久改变，这可能会产生有害的后果。证明膳食补充抗氧化剂可以减少氧化 DNA 损伤，可以为这种补充在预防癌症和与年龄相关的疾病方面的价值提供证据。
本研究旨在探讨番茄汁是否能防止未经训练的个体因剧烈运动而产生的 ROS。作为氧化应激的标志物，使用改良的 ELISA 监测血清 8-oxodG 水平。对 15 名未经训练的健康受试者进行了干预，他们使用自行车功量计以 80%的最大脉搏进行 20 分钟的运动。在运动前和运动后 1 小时采集血样。5 周后，每天摄入 150 毫升番茄汁，然后进行 5 周的洗脱期，再进行 5 周的番茄汁摄入。结果表明，每天摄入相当于 15 毫克番茄红素的番茄汁 5 周，可显著降低剧烈运动后血清 8-oxodG 水平。
这些数据强烈表明，番茄汁具有潜在的抗氧化作用，可能降低氧化应激引起的 ROS 水平升高。
