Haghdoost Siamak, Maruyama Yukio, Pecoits-Filho Roberto, Heimburger Olof, Seeberger Astrid, Anderstam Björn, Suliman Mohamed E, Czene Stefan, Lindholm Bengt, Stenvinkel Peter, Harms-Ringdahl Mats
Department of Genetics, Microbiology and Toxicology, Stockholm University, Stockholm, Sweden.
Antioxid Redox Signal. 2006 Nov-Dec;8(11-12):2169-73. doi: 10.1089/ars.2006.8.2169.
Does inflammation, as assessed by high sensitivity C-reactive protein (hs-CRP), in patients with end-stage renal disease (ESRD) tightly associate with increased serum levels of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8- oxo-dG)? Increased oxidative stress and inflammation have both been highlighted among several nontraditional risk factors for cardiovascular disease, which is the main cause of mortality in ESRD patients. In contrast to oxidative stress effects on proteins and lipids, DNA base damage has not been well demonstrated in ESRD. Two groups of hemodialysis patients were studied, one group with persistent inflammation (n = 13, with constant elevation of CRP > 10 mg/L for 6 months) and one group of noninflamed patients (n = 19, with constant CRP < 10 mg/L for 6 months). Serum 8-oxo-dG was significantly elevated in persistent inflammation in comparison to noninflamed patients. At an individual level, a significant correlation was found between serum 8-oxo-dG and hsCRP. Extracellular 8-oxo-dG leads to intracellular oxidative damage on the nucleotide pool, thus providing a sensitive marker for inflammatory response. Serum levels of 8-oxo-dG, in combination with other inflammatory markers, serve as useful diagnostic tools for identification of patients in risk for inflammatory complications.
终末期肾病（ESRD）患者中，通过高敏C反应蛋白（hs-CRP）评估的炎症与血清8-氧代-7,8-二氢-2'-脱氧鸟苷（8-氧代-dG）水平升高是否密切相关？氧化应激增加和炎症在心血管疾病的多种非传统危险因素中都很突出，而心血管疾病是ESRD患者死亡的主要原因。与氧化应激对蛋白质和脂质的影响不同，DNA碱基损伤在ESRD中尚未得到充分证实。研究了两组血液透析患者，一组为持续性炎症患者（n = 13，CRP持续升高> 10 mg/L达6个月），另一组为非炎症患者（n = 19，CRP持续< 10 mg/L达6个月）。与非炎症患者相比，持续性炎症患者的血清8-氧代-dG显著升高。在个体水平上，血清8-氧代-dG与hsCRP之间存在显著相关性。细胞外8-氧代-dG会导致核苷酸池的细胞内氧化损伤，从而为炎症反应提供一个敏感的标志物。血清8-氧代-dG水平与其他炎症标志物相结合，可作为识别有炎症并发症风险患者的有用诊断工具。
