Sakai Hiroki, Murakami Mami, Mishima Hiroyuki, Hoshino Yuki, Mori Takashi, Maruo Kohji, Yanai Tokuma
Laboratory of Veterinary Pathology, Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan.
Vet Clin Pathol. 2012 Jun;41(2):291-4. doi: 10.1111/j.1939-165X.2012.00430.x. Epub 2012 May 2.
A 10-year-old intact female Shetland Sheepdog with tenesmus had a subcutaneous mass at the left ventral aspect of the anus. On cytologic examination, 2 types of cells were observed. Most of the cells were oval to polygonal and had elliptical or elongate nuclei and a moderate amount of pale to basophilic cytoplasm. The remaining cells had round to oval nuclei and pale to basophilic cytoplasm. Cells of both types were loosely adhered to each other and were arranged in rosette-like structures. Both neoplastic cell types had fine homogenous chromatin and either a small indistinct nucleolus or no visible nucleolus. Mild anisokaryosis and anisocytosis were observed. Histologically, the mass consists of glandular structures formed by cuboidal cells admixed with bundles of spindle cells. Eosinophilic PAS- and Alcian blue-positive secretory material was found in the center of some glandular structures. Both neoplastic cell types had positive staining with paradoxical concanavalin A and expressed cytokeratin, but not vimentin, S-100, α-smooth muscle actin, or desmin. Based on location and histologic and immunohistochemical features, the final diagnosis was adenocarcinoma of the apocrine gland of the anal sac, which should be included as a cytologic differential diagnosis when spindle cells and typical epithelial cells are observed in masses in the region of the anal sac of dogs.
一只10岁未绝育的雌性设得兰牧羊犬，有里急后重症状，在肛门左侧腹面有一个皮下肿块。在细胞学检查中，观察到两种类型的细胞。大多数细胞呈椭圆形至多边形，有椭圆形或细长的细胞核以及中等量的淡嗜碱性至嗜碱性细胞质。其余细胞有圆形至椭圆形的细胞核和淡嗜碱性至嗜碱性细胞质。两种类型的细胞彼此松散黏附，并排列成玫瑰花结样结构。两种肿瘤细胞类型都有细的均匀染色质，要么有一个小的不明显核仁，要么没有可见核仁。观察到轻度核大小不等和细胞大小不等。组织学上，肿块由立方体细胞形成的腺结构与梭形细胞束混合组成。在一些腺结构的中心发现了嗜酸性过碘酸雪夫染色和阿尔辛蓝阳性的分泌物质。两种肿瘤细胞类型对反常伴刀豆球蛋白A染色均为阳性，并表达细胞角蛋白，但不表达波形蛋白、S-100、α-平滑肌肌动蛋白或结蛋白。根据位置以及组织学和免疫组化特征，最终诊断为肛门囊顶泌汗腺腺癌，当在犬肛门囊区域的肿块中观察到梭形细胞和典型上皮细胞时，应将其作为细胞学鉴别诊断之一。
