Department of Orthopaedic Surgery, Surgical Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Biomaterials. 2012 Jul;33(21):5278-86. doi: 10.1016/j.biomaterials.2012.03.073. Epub 2012 Apr 30.
In this study, we investigate the effects of treatment with layered chondrocyte sheets and synovial cell transplantation. An osteochondral defect was created of 48 Japanese white rabbits. In order to determine the effects of treatment, the following 6 groups were produced: (A) synovial cells (1.8 × 10(6) cells), (B)layered chondrocyte sheets (1.7 × 10(6) cells), (C) synovial cells (3.0 × 10(5) cells) + layered chondrocyte sheets, (D)synovial cells (6.0 × 10(5) cells) + layered chondrocyte sheets, (E)synovial cells (1.2 × 10(6) cells) + layered chondrocyte sheets, (F) osteochondral defect. Layered chondrocyte sheets and synovial cells were transplanted, sacrificed four and 12 weeks postoperatively. An incapacitance tester (Linton) was used to find trends in the weight distribution ratio of the damaged limbs after surgery. Sections were stained with Safranin-O. Repair sites were evaluated using ICRS grading system. In groups (A) to (E), the damaged limb weight distribution ratio had improved. The repair tissue stained positively with Safranin-O. Four and 12 weeks after surgery, groups (A) to (E) exhibited significantly higher scores than group (F), and groups (D) and (E) exhibited significantly higher scores than groups (A) and (B). This suggests the efficacy of combining layered chondrocyte sheets with synovial cells.
在这项研究中，我们研究了治疗分层软骨细胞片和滑膜细胞移植的效果。在 48 只日本白兔中创建了一个软骨下骨缺损。为了确定治疗效果，产生了以下 6 个组：（A）滑膜细胞（1.8×10(6)个细胞），（B）分层软骨细胞片（1.7×10(6)个细胞），（C）滑膜细胞（3.0×10(5)个细胞）+分层软骨细胞片，（D）滑膜细胞（6.0×10(5)个细胞）+分层软骨细胞片，（E）滑膜细胞（1.2×10(6)个细胞）+分层软骨细胞片，（F）软骨下骨缺损。分层软骨细胞片和滑膜细胞被移植，术后 4 周和 12 周处死。使用（Linton）测功机寻找手术后患肢重量分布比例的趋势。使用番红 O 染色进行染色。使用 ICRS 分级系统评估修复部位。在（A）到（E）组中，受损肢体的重量分布比例有所改善。番红 O 染色阳性的修复组织。手术后 4 周和 12 周，（A）到（E）组的评分明显高于（F）组，（D）组和（E）组的评分明显高于（A）组和（B）组。这表明将分层软骨细胞片与滑膜细胞结合的效果。
