Department of Dermatology, Chonnam National University Medical School, 671 Jebongro, Donggu, Gwangju, Republic of Korea.
J Dermatol Sci. 2012 Jul;67(1):3-8. doi: 10.1016/j.jdermsci.2012.04.001. Epub 2012 Apr 12.
Malassezia (M.) species are members of the normal part of the skin flora, but they might induce or be involved with various cutaneous diseases. Although the role of Malassezia in the pathogenesis of cutaneous diseases is not fully understood, recent studies have shown that decreased density of Malassezia led to improvement of these diseases.
To identify the antifungal effect of light emitting diode (LED) against Malassezia, its antifungal mechanisms and the impact on the keratinocytes.
LED with various wavelengths (370-630nm) on Malassezia furfur, Malassezia sympodialis and Malassezia globosa was irradiated according to dose and then the antifungal effects were thereafter assessed. After irradiating LED with 392.5±1nm of wavelength according to dose on Malassezia species, reactive oxygen species (ROS) and lipid hydroperoxide production assay were measured. In addition, cell viability and inflammatory cytokines (IL-1α, IL-1β, TNF-α, TGF-β, TLR-2 and COX-2) expressions in normal human epidermal keratinocytes (NHEKs) by LED irradiation were evaluated.
The growth of Malassezia species was dose-dependently suppressed by both LED with 380±2 and 392.5±1nm wavelengths. The increases of intracellular and extracellular ROS by LED irradiation with 392.5±1nm wavelengths were significantly observed compared to control group. The cell viability and cytokines in NHEKs were not significantly affected by LED irradiation under 5J/cm(2)in vitro.
LED irradiation with 380±2 and 392.5±1nm wavelengths proved to have antifungal effect against Malassezia species and no impact on NHEKs under 5J/cm(2). The findings suggest that LED might be an adjunctive therapeutic light tool against Malassezia yeasts related cutaneous diseases.
马拉色菌（M.）是皮肤正常菌群的一部分，但它们可能会引起或参与各种皮肤疾病。尽管马拉色菌在皮肤疾病发病机制中的作用尚未完全了解，但最近的研究表明，马拉色菌密度的降低导致这些疾病的改善。
确定发光二极管（LED）对马拉色菌的抗真菌作用、其抗真菌机制以及对角质形成细胞的影响。
根据剂量照射具有不同波长（370-630nm）的 LED 于糠秕马拉色菌、合轴马拉色菌和球形马拉色菌上，然后评估抗真菌效果。根据剂量用波长 392.5±1nm 的 LED 照射马拉色菌后，测量活性氧（ROS）和脂质过氧化物的产生。此外，评估 LED 照射对正常人体表皮角质形成细胞（NHEK）中细胞活力和炎症细胞因子（IL-1α、IL-1β、TNF-α、TGF-β、TLR-2 和 COX-2）表达的影响。
波长为 380±2nm 和 392.5±1nm 的 LED 均能剂量依赖性地抑制马拉色菌的生长。与对照组相比，LED 照射后细胞内和细胞外 ROS 的增加明显。在体外 5J/cm(2) 下，LED 照射对 NHEK 细胞活力和细胞因子无明显影响。
波长为 380±2nm 和 392.5±1nm 的 LED 对马拉色菌具有抗真菌作用，在 5J/cm(2) 下对 NHEK 无影响。这些发现表明，LED 可能成为治疗与马拉色菌相关的皮肤疾病的辅助治疗光工具。
