Department of Urology, The Fourth Hospital, Hebei Medical University, Hebei 050011, PR China.
Oncol Rep. 2012 Jul;28(1):85-90. doi: 10.3892/or.2012.1783. Epub 2012 Apr 23.
Curcumin (CUR) is a natural agent that has been demonstrated to effectively inhibit prostate cancer growth. However, natural CUR is relatively unstable and can be easily degraded in vivo. Therefore, it is essential to develop other stable curcuminoids. Demethoxycurcumin (DMC) is a candidate that has been verified in several tumor types and has potential for the treatment of prostate cancer. In the present study, we investigated the effects of DMC on proliferation, apoptosis and migration of PC-3 cells. MTT assay results indicated that DMC inhibited PC-3 cell viability in a dose- and time-dependent manner, and DMC induced G2/M phase arrest. Furthermore, PC-3 cells in DMC-treated groups had a higher apoptotic rate compared with DMSO-treated control. This effect may be due to the activation of the caspase-3 pathway. In DMC-treated groups, migrating and invasive cells were dramatically reduced (P<0.05). The activity of MMP-2, which is correlated with migration and invasion was also suppressed by DMC. These results indicated that DMC may inhibit PC-3 cell migration and invasion partially by affecting MMP-2 activity. In conclusion, DMC significantly inhibits proliferation, migration and invasion of cultured PC-3 cells, and this study may provide evidence for future in vivo studies and clinical use.
姜黄素（CUR）是一种已被证实能有效抑制前列腺癌生长的天然药物。然而，天然 CUR 相对不稳定，在体内容易降解。因此，开发其他稳定的姜黄素类似物至关重要。去甲氧基姜黄素（DMC）是一种已在多种肿瘤类型中得到验证的候选药物，具有治疗前列腺癌的潜力。在本研究中，我们研究了 DMC 对 PC-3 细胞增殖、凋亡和迁移的影响。MTT 试验结果表明，DMC 呈剂量和时间依赖性抑制 PC-3 细胞活力，且诱导 G2/M 期阻滞。此外，与 DMSO 处理对照组相比，DMC 处理组的 PC-3 细胞凋亡率更高。这种作用可能是由于 caspase-3 途径的激活。在 DMC 处理组中，迁移和侵袭的细胞明显减少（P<0.05）。与迁移和侵袭相关的 MMP-2 的活性也被 DMC 抑制。这些结果表明，DMC 可能通过影响 MMP-2 活性部分抑制 PC-3 细胞的迁移和侵袭。总之，DMC 显著抑制培养的 PC-3 细胞的增殖、迁移和侵袭，本研究可为未来的体内研究和临床应用提供依据。
