Torres Rogil José de Almeida, Noronha Lucia de, Casella Antonio Marcelo Barbante, Lumikoski Thaís Isabel, Précoma Leonardo Brandão, Torres Caroline Luzia de Almeida, Luchini Andréa, Sturzeneker Mario Claudio Soares, Précoma Dalton Bertolim
Pontifícia Universidade Católica do Paraná, Curitiba, PR, Brazil.
Arq Bras Oftalmol. 2012 Jan-Feb;75(1):48-52. doi: 10.1590/s0004-27492012000100010.
The aim of this study is to experientially demonstrate that a cholesterol-enriched diet induces an increase in the MCP-1 expression in the choroid and sclera.
New Zealand rabbits were divided into two groups: GN (normal diet group) of 8 rabbits (8 eyes) was fed a standard rabbit diet for 4 weeks; GH (hypercholesterolemic group) of 13 rabbits (13 eyes) was fed a 1% cholesterol enriched diet for 8 weeks. Total serum cholesterol, triglyceride, HDL cholesterol and fasting blood glucose exams were performed at the start of the experiment and at the euthanasia time. After GH 8th week and GN 4th week animals were euthanized and their eyes underwent immunohistochemical analysis with the anti-MCP-1 antibody.
The diet has induced a significant increase in GH total cholesterol and triglyceride levels when compared with NG (p<0.001). There was a significant increase in the MCP-1 expression in GH choroid and sclera in relation to GN (p<0.001).
This study has revealed that the hypercholesterolemic diet in rabbits induces an increase in the MCP-1 expression in the choroid and sclera.
本研究旨在通过实验证明，高胆固醇饮食会导致脉络膜和巩膜中MCP-1表达增加。
将新西兰兔分为两组：8只兔子（8只眼）的GN组（正常饮食组）喂食标准兔粮4周；13只兔子（13只眼）的GH组（高胆固醇血症组）喂食含1%胆固醇的饮食8周。在实验开始时和安乐死时进行总血清胆固醇、甘油三酯、高密度脂蛋白胆固醇和空腹血糖检查。在GH组第8周和GN组第4周后，对动物实施安乐死，并使用抗MCP-1抗体对其眼睛进行免疫组织化学分析。
与NG组相比，该饮食使GH组的总胆固醇和甘油三酯水平显著升高（p<0.001）。与GN组相比，GH组脉络膜和巩膜中的MCP-1表达显著增加（p<0.001）。
本研究表明，兔的高胆固醇饮食会导致脉络膜和巩膜中MCP-1表达增加。
