Torres Rogil José de Almeida, Luchini Andrea, Both Alessandro Soares, Precoma Leonardo Brandao, Champoski Ana Flavia, Schuh Maynara Leonardi, Torres Renan Pedro de Almeida, Noronha Lucia, Pessoa Bernardo Molinari, Torres Lucas Antonio de Almeida, Serci Nicole Francesca de França, Precoma Dalton Bertolim
Department of Opthalmology,Pontificia Universidade Catolica do Parana,Rua Emiliano Perneta 390, Conj 1407,CEP 80420-080,Curitiba,Parana,Brazil.
Centro Oftalmologico de Curitiba,Curitiba,Parana,Brazil.
Br J Nutr. 2014 Nov 14;112(9):1438-46. doi: 10.1017/S0007114514002463. Epub 2014 Sep 23.
The aim of the present study was to evaluate the effect of flaxseed on choroid-sclera complex thickness and on LDL oxidation in the sclera, choroid and retina of diet-induced hypercholesterolaemic rabbits. New Zealand male albino rabbits (n 21) were divided into two groups: group 1 (G1; n 11), fed a hypercholesterolaemic diet, and group 2 (G2; n 10), fed a hypercholesterolaemic diet enriched with flaxseed flour. The serum concentrations of total cholesterol (TC), LDL-cholesterol (LDL-C), HDL-cholesterol, TAG and fasting blood glucose were determined at the start of the experiment and on the day of killing (8th week). Choroid and sclera samples were subjected to haematoxylin-eosin (HE) staining and histomorphometric and immunohistochemical analyses with the anti-oxidised LDL antibody. Sensory retina samples were subjected to an immunohistochemical analysis with the primary monoclonal nitrotyrosine antibody. At the end of the experiment, a significant increase was observed in TC and LDL-C concentrations in G1 rabbits when compared with G2 rabbits (P= 0·008 and P= 0·02, respectively). HE staining revealed a significant increase in choroid-sclera complex thickness in G1 rabbits when compared with G2 rabbits (P< 0·001). Immunohistochemical analysis of choroid and sclera samples with the anti-oxidised LDL marker revealed a significant increase in immunoreactivity in G1 rabbits when compared with G2 rabbits (P< 0·001). Immunohistochemical analysis of sensory retina samples with the anti-nitrotyrosine marker revealed a significant increase in immunoreactivity in G1 rabbits when compared with G2 rabbits (P= 0·002). Flaxseed reduced the choroid-sclera complex thickness of diet-induced hypercholesterolaemic rabbits and the expression of oxidised LDL in the choroid-sclera complex as well as the expression of nitrotyrosine in the sensory retina.
本研究的目的是评估亚麻籽对饮食诱导的高胆固醇血症兔脉络膜 - 巩膜复合体厚度以及巩膜、脉络膜和视网膜中低密度脂蛋白（LDL）氧化的影响。将21只新西兰雄性白化兔分为两组：第1组（G1，n = 11），喂食高胆固醇血症饮食；第2组（G2，n = 10），喂食富含亚麻籽粉的高胆固醇血症饮食。在实验开始时和处死当天（第8周）测定血清总胆固醇（TC）、低密度脂蛋白胆固醇（LDL - C）、高密度脂蛋白胆固醇、甘油三酯（TAG）和空腹血糖浓度。脉络膜和巩膜样本进行苏木精 - 伊红（HE）染色，并使用抗氧化LDL抗体进行组织形态计量学和免疫组织化学分析。感觉视网膜样本使用抗硝基酪氨酸单克隆抗体进行免疫组织化学分析。实验结束时，与G2组兔相比，G1组兔的TC和LDL - C浓度显著升高（分别为P = 0.008和P = 0.02）。HE染色显示，与G2组兔相比，G1组兔的脉络膜 - 巩膜复合体厚度显著增加（P < 0.001）。用抗氧化LDL标记物对脉络膜和巩膜样本进行免疫组织化学分析显示，与G2组兔相比，G1组兔的免疫反应性显著增加（P < 0.001）。用抗硝基酪氨酸标记物对感觉视网膜样本进行免疫组织化学分析显示，与G2组兔相比，G1组兔的免疫反应性显著增加（P = 0.002）。亚麻籽降低了饮食诱导的高胆固醇血症兔的脉络膜 - 巩膜复合体厚度以及脉络膜 - 巩膜复合体中氧化LDL的表达和感觉视网膜中硝基酪氨酸的表达。
