1 Pontificia Universidade Catolica do Parana , Curitiba, Brazil .
J Ocul Pharmacol Ther. 2013 Oct;29(8):709-14. doi: 10.1089/jop.2012.0142. Epub 2013 May 17.
Demonstrate that the blockade of angiotensin II AT-1 receptors, through the systemic administration of olmesartan, can reduce the MCP-1 expression and the resulting macrophage accumulation in the choroid and sclera of hypercholesterolemic rabbits.
Thirty-two New Zealand rabbits were divided into 3 groups: group I (GI) was fed a standard rabbit diet; group II (GII) was fed a hypercholesterolemic diet; and group III (GIII) was fed a hypercholesterolemic diet plus olmesartan. Serum levels of total cholesterol, triglyceride, HDL cholesterol, and blood glucose were determined in fasting rabbits at the beginning of the experiment and on the day of euthanasia. The choroid and sclera were submitted to morphometric analysis as well as immunohistochemical analysis with MCP-1 and RAM-11 (macrophage marker) antibodies.
No abnormality was detected in GI. Group II and III had significant increases in choroid-sclera complex thicknesses when compared with group I (P<0.001). GII showed a significant increase in immunoreactivity for MCP-1 in relation to GI (P=0.001) and GIII (P=0.004). GII showed a significant increase in immunoreactivity for RAM-11 of the choroid-sclera complex in relation to GI (P<0.001) and GIII (P=0.034). A significant increase in immunoreactivity for RAM-11 was observed in GIII in relation to GI (P=0.008).
Olmesartan reduced the MCP-1 expression and the resultant macrophage accumulation in the choroid-sclera complex of hypercholesterolemic rabbits.
通过全身给予奥美沙坦阻断血管紧张素 II AT-1 受体，证明其可减少高胆固醇血症兔脉络膜和巩膜中单核细胞趋化蛋白-1（MCP-1）的表达和由此导致的巨噬细胞积聚。
32 只新西兰兔随机分为 3 组：I 组（GI）给予标准兔饲料；II 组（GII）给予高胆固醇饲料；III 组（GIII）给予高胆固醇饲料加奥美沙坦。实验开始和处死当天，禁食的兔子检测血清总胆固醇、甘油三酯、高密度脂蛋白胆固醇和血糖水平。对脉络膜和巩膜进行形态计量学分析以及 MCP-1 和 RAM-11（巨噬细胞标志物）抗体免疫组织化学分析。
GI 组无异常。与 GI 组相比，GII 和 GIII 组的脉络膜-巩膜复合体厚度均显著增加（P<0.001）。与 GI 组（P=0.001）和 GIII 组（P=0.004）相比，GII 组 MCP-1 免疫反应性显著增加。与 GI 组（P<0.001）和 GIII 组（P=0.034）相比，GII 组脉络膜-巩膜复合体中 RAM-11 的免疫反应性显著增加。与 GI 组相比，GIII 组 RAM-11 的免疫反应性显著增加（P=0.008）。
奥美沙坦可减少高胆固醇血症兔脉络膜-巩膜复合体中 MCP-1 的表达和由此导致的巨噬细胞积聚。
