Padilla E Carlos, Núñez A María, Padilla G Andrés, Lobos G Olga
Departamento de Microbiología, Universidad de Talca, Talca, Chile.
Rev Chilena Infectol. 2012 Feb;29(1):55-61. doi: 10.4067/S0716-10182012000100010. Epub 2012 Apr 10.
The presence of virulence genes (VG) and bacteriocins from different clinical samples was studied in Enterococcus faecalis isolated from urinary tract infections (UTI), bacteremia and endodontitis and was correlated with haemolysin and gelatinase activity. We evaluated the presence of VG by PCR in 150 strains of E. faecalis including cylA, aggA, efaA, eep, gelE, esp, as-48, bac31, entL50A/B, entA, entP, entB, enlA andentl071. Haemolysin and gelatinase activity was studied. gelE and cylA genes expressed hemolysin and gelatinase, respectively. This activity was observed in some strains of bacteremia, UTI and endodontitis. The highest number of VG was detected in bacteremic strains, being aggA and entA genes the most frequent. efaA, esp, entA, entL50A/B were associated with their clinical origin (p < 0.05). The most common genetic profile was aggA-eep-enlA-entL50A/B. E. faecalis from UTI, bacteremia and endodontitis presented different gene combinations. Some of the genes studied were related to their clinical origin. The results obtained in this study are similar to those reported in other countries.
对从尿路感染(UTI)、菌血症和牙髓炎中分离出的粪肠球菌不同临床样本中的毒力基因(VG)和细菌素进行了研究,并将其与溶血素和明胶酶活性相关联。我们通过PCR评估了150株粪肠球菌中VG的存在情况,包括cylA、aggA、efaA、eep、gelE、esp、as-48、bac31、entL50A/B、entA、entP、entB、enlA和entl071。研究了溶血素和明胶酶活性。gelE和cylA基因分别表达溶血素和明胶酶。在一些菌血症、UTI和牙髓炎菌株中观察到了这种活性。在菌血症菌株中检测到的VG数量最多,其中aggA和entA基因最为常见。efaA、esp、entA、entL50A/B与其临床来源相关(p<0.05)。最常见的基因谱是aggA-eep-enlA-entL50A/B。来自UTI、菌血症和牙髓炎的粪肠球菌呈现出不同的基因组合。所研究的一些基因与其临床来源有关。本研究获得的结果与其他国家报道的结果相似。