Seno Yuko, Kariyama Reiko, Mitsuhata Ritsuko, Monden Koichi, Kumon Hiromi
Department of Urology, Okayama University Graduate School of Medicine and Dentistry, Okayama 700--8558, Japan.
Acta Med Okayama. 2005 Jun;59(3):79-87. doi: 10.18926/AMO/31979.
The potential relationships between biofilm formation and pathogenicity of Enterococcus faecalis in urinary tract infections (UTI) were investigated. Over a 12-year period from 1991 through 2002, a total of 352 E.faecalis isolates were collected from patients with complicated UTI (one isolate per patient) at the urology ward of Okayama University Hospital. We analyzed the prevalence and transferability of genes encoding virulence factors(asa1, esp, cylA, gelE /sprE )and antimicrobial resistance(aac(6') /aph(2'')). The production of biofilm, hemolysin and gelatinase by these isolates was also examined and the associated medical records of patients were retrospectively reviewed. Of 352 E. faecalis isolates, 315 possessed and/or genes. Of the 63 hemolysin- and 167 gelatinase-producing isolates, 59 and 94 isolates, respectively, possessed both asa1 and esp genes. E. faecalis isolates with both asa1 and esp genes formed biofilms at significantly higher rates than those with neither gene (P=0.038). The genes encoding asa1, cylA , and aac(6') /(aph(2'') were transferable and appeared to have accumulated in these isolates. The E. faecalis isolates possessing asa1 and/or esp genes were found from both catheter-related or -unrelated UTI. Our study indicates that E. faecalis isolates that have accumulated virulence genes are apt to form persistent biofilms in the urinary tracts.
研究了粪肠球菌在尿路感染（UTI）中生物膜形成与致病性之间的潜在关系。在1991年至2002年的12年期间，从冈山大学医院泌尿外科病房的复杂性UTI患者中总共收集了352株粪肠球菌分离株（每位患者一株）。我们分析了编码毒力因子（asa1、esp、cylA、gelE / sprE）和抗菌抗性（aac(6') / aph(2'')）的基因的流行率和可转移性。还检测了这些分离株生物膜、溶血素和明胶酶的产生情况，并对患者的相关病历进行了回顾性审查。在352株粪肠球菌分离株中，315株拥有和/或基因。在63株产生溶血素和167株产生明胶酶的分离株中，分别有59株和94株同时拥有asa1和esp基因。同时拥有asa1和esp基因的粪肠球菌分离株形成生物膜的比例明显高于两者都没有的分离株（P = 0.038）。编码asa1、cylA和aac(6') / (aph(2''))的基因是可转移的，并且似乎在这些分离株中积累。在与导管相关或不相关的UTI中均发现了拥有asa1和/或esp基因的粪肠球菌分离株。我们的研究表明，积累了毒力基因的粪肠球菌分离株易于在尿路中形成持久性生物膜。
