使用吖啶橙染色的血沉棕黄层涂片快速诊断成人菌血症。
Rapid diagnosis of bacteremia in adults using acridine orange stained buffy coat smears.
作者信息
Miller M, Mendelson J
机构信息
Department of Microbiology, McGill University; and Division of Infectious Diseases (Department of Medicine), Jewish General Hospital, Montreal, Quebec.
出版信息
Can J Infect Dis. 1990 Spring;1(1):7-10. doi: 10.1155/1990/159215.
The use of acridine orange stained buffy coat smears was assessed as a rapid screening test for bacteremia in adults. A total of 356 consecutive blood cultures were submitted with simultaneous anticoagulated blood samples, from which a buffy coat smear was prepared and stained with acridine orange (100 mg/L; pH 3.0). Forty-one of 356 blood samples (12%) yielded organisms in the blood culture system. Compared to blood culture, the overall sensitivity of acridine orange stained buffy coat smears was 16%, specificity 88%, and positive predictive value 13%. There was no statistically significant difference in performance of the test among patients who had fever greater than 39°C and/or shock. The low sensitivity and specificity of the test makes it unsuitable as a means of rapid screening for adults with suspected bacteremia.
评估吖啶橙染色的血沉棕黄层涂片作为成人菌血症快速筛查试验的效果。共提交了356份连续血培养样本及同时采集的抗凝血液样本，从中制备血沉棕黄层涂片并用吖啶橙（100 mg/L；pH 3.0）染色。356份血样本中有41份（12%）在血培养系统中检出微生物。与血培养相比，吖啶橙染色的血沉棕黄层涂片总体敏感性为16%，特异性为88%，阳性预测值为13%。在体温高于39°C和/或休克的患者中，该试验的表现无统计学显著差异。该试验的低敏感性和特异性使其不适用于作为疑似菌血症成人的快速筛查手段。
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