Mascart G, Bertrand F, Mascart P
J Clin Pathol. 1983 May;36(5):595-7. doi: 10.1136/jcp.36.5.595.
In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances and after 48 h in 525. We were able to demonstrate the value of direct examination. Staining with acridine orange yields more positive results than Gram staining and is also simpler.
鉴于败血症快速病因诊断的重要性，我们比较了亚培养、革兰氏染色和吖啶橙染色在检测阳性血培养物中的结果。该研究基于1013份血培养物，其中138份经培养呈阳性。在210例中，标本采集后12小时应用这三种技术，540例在24小时应用，525例在48小时后应用。我们能够证明直接检查的价值。吖啶橙染色比革兰氏染色产生更多阳性结果，并且也更简单。
