亚培养、革兰氏染色和吖啶橙染色用于早期检测阳性血培养物的比较研究。
Comparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.
作者信息
Mascart G, Bertrand F, Mascart P
出版信息
J Clin Pathol. 1983 May;36(5):595-7. doi: 10.1136/jcp.36.5.595.
Abstract
In view of the importance of a rapid aetiological diagnosis in septicaemia, we compared the results of subculture, Gram staining and acridine orange staining in the detection of positive blood cultures. The study was based on 1013 blood cultures of which 138 were positive by culture. The three techniques were applied 12 h after the specimen was taken in 210 instances, at 24 h in 540 instances and after 48 h in 525. We were able to demonstrate the value of direct examination. Staining with acridine orange yields more positive results than Gram staining and is also simpler.
摘要
鉴于败血症快速病因诊断的重要性，我们比较了亚培养、革兰氏染色和吖啶橙染色在检测阳性血培养物中的结果。该研究基于1013份血培养物，其中138份经培养呈阳性。在210例中，标本采集后12小时应用这三种技术，540例在24小时应用，525例在48小时后应用。我们能够证明直接检查的价值。吖啶橙染色比革兰氏染色产生更多阳性结果，并且也更简单。
相似文献
1
Comparative study of subculture, Gram staining and acridine orange staining for early detection of positive blood cultures.亚培养、革兰氏染色和吖啶橙染色用于早期检测阳性血培养物的比较研究。
J Clin Pathol. 1983 May;36(5):595-7. doi: 10.1136/jcp.36.5.595.
2
Acridine orange stain in the early detection of bacteria in blood cultures.吖啶橙染色在血培养中细菌早期检测中的应用
Eur J Clin Microbiol. 1984 Apr;3(2):113-5. doi: 10.1007/BF02014327.
3
Detection of bacteremia by buffy coat smears.通过血沉棕黄层涂片检测菌血症。
Scand J Infect Dis. 1987;19(2):215-7. doi: 10.3109/00365548709032401.
4
Differential fluorescent staining method for detection of bacteria in blood cultures, cerebrospinal fluid and other clinical specimens.用于检测血培养、脑脊液及其他临床标本中细菌的差异荧光染色法。
Eur J Clin Microbiol Infect Dis. 2002 May;21(5):373-8. doi: 10.1007/s10096-002-0731-3. Epub 2002 May 17.
5
Blood culture gram stain, acridine orange stain and direct sensitivity-based antimicrobial therapy of bloodstream infection in patients with trauma.创伤患者血流感染的血培养革兰氏染色、吖啶橙染色及基于直接药敏的抗菌治疗
Indian J Med Microbiol. 2010 Apr-Jun;28(2):138-42. doi: 10.4103/0255-0857.62491.
6
Utility of Acridine Orange staining for detection of bacteria from positive blood cultures.吖啶橙染色在检测阳性血培养物中细菌的应用。
J Microbiol Methods. 2017 Aug;139:215-217. doi: 10.1016/j.mimet.2017.06.014. Epub 2017 Jun 15.
7
Rapid detection of bacteraemia by early subculture.通过早期传代培养快速检测菌血症
J Med Microbiol. 1984 Jun;17(3):311-5. doi: 10.1099/00222615-17-3-311.
8
Subcultures of BACTEC-positive but gram or acridine orange stain-negative NR 6A and 7A blood culture bottles are unnecessary.对BACTEC阳性但革兰氏或吖啶橙染色阴性的NR 6A和7A血培养瓶进行传代培养并无必要。
Diagn Microbiol Infect Dis. 1994 Nov;20(3):121-5. doi: 10.1016/0732-8893(94)90105-8.
9
Rapid screening of urine for significant bacteriuria by Gram stain, acridine orange stain, and the Autobac MTS system.通过革兰氏染色、吖啶橙染色和自动细菌鉴定药敏分析系统对尿液进行显著菌尿的快速筛查。
Diagn Microbiol Infect Dis. 1984 Jun;2(3):179-86. doi: 10.1016/0732-8893(84)90028-2.
10
Evaluation of acridine orange stain for detection of microorganisms in blood cultures.吖啶橙染色用于检测血培养中微生物的评估。
J Clin Microbiol. 1980 Mar;11(3):281-5. doi: 10.1128/jcm.11.3.281-285.1980.
引用本文的文献
1
Rapid diagnosis of bacteremia in adults using acridine orange stained buffy coat smears.使用吖啶橙染色的血沉棕黄层涂片快速诊断成人菌血症。
Can J Infect Dis. 1990 Spring;1(1):7-10. doi: 10.1155/1990/159215.
2
Use of Directigen and acridine orange for rapid identification of human blood culture isolates.使用直接igen和吖啶橙快速鉴定人血培养分离株。
J Clin Microbiol. 1984 Sep;20(3):587-8. doi: 10.1128/jcm.20.3.587-588.1984.
3
Controlled evaluation of supplemented peptone and Bactec blood culture broths for the detection of bacteremia and fungemia.补充蛋白胨和Bactec血培养肉汤用于检测菌血症和真菌血症的对照评估。
J Clin Microbiol. 1985 Apr;21(4):531-4. doi: 10.1128/jcm.21.4.531-534.1985.
4
Current controversies in the detection of septicemia.败血症检测中的当前争议
Eur J Clin Microbiol. 1986 Oct;5(5):487-91. doi: 10.1007/BF02017688.
本文引用的文献
1
Comparison of a slide blood culture system with a supplemented peptone broth culture method.玻片血培养系统与补充蛋白胨肉汤培养法的比较。
J Clin Microbiol. 1981 Oct;14(4):389-92. doi: 10.1128/jcm.14.4.389-392.1981.
2
Evaluation of acridine orange stain for detection of microorganisms in blood cultures.吖啶橙染色用于检测血培养中微生物的评估。
J Clin Microbiol. 1980 Mar;11(3):281-5. doi: 10.1128/jcm.11.3.281-285.1980.
3
Automated radiometric detection of bacterial growth in blood cultures.血培养中细菌生长的自动放射性检测
J Lab Clin Med. 1970 Mar;75(3):529-34.
4
Comparison of two liquid blood culture media containing sodium polyanethole sulfonate: tryptic soy and Columbia.两种含聚茴香脑磺酸钠的液体血液培养基的比较：胰蛋白酶大豆培养基和哥伦比亚培养基。
Appl Microbiol. 1974 Apr;27(4):699-702. doi: 10.1128/am.27.4.699-702.1974.
5
Evaluation of radiometric system for detecting bacteremia.用于检测菌血症的辐射测量系统的评估
Appl Microbiol. 1973 Sep;26(3):368-72. doi: 10.1128/am.26.3.368-372.1973.
6
Rapid detection of bacteremia by a radiometric system. A clinical evaluation.用放射性测量系统快速检测菌血症。临床评估。
Am J Clin Pathol. 1974 Jun;61(6):859-66. doi: 10.1093/ajcp/61.6.859.
7
Comparison of macroscopic examination, routine gram stains, and routine subcultures in the initial detection of positive blood cultures.在血培养阳性的初始检测中，宏观检查、常规革兰氏染色和常规传代培养的比较。
Appl Microbiol. 1974 Mar;27(3):537-9. doi: 10.1128/am.27.3.537-539.1974.
8
New centrifugation blood culture device.新型离心式血液培养装置。
J Clin Microbiol. 1978 Jan;7(1):52-4. doi: 10.1128/jcm.7.1.52-54.1978.
Zotero 插件