(R,R)-XY-10和(S,S)-XY-10诱导视网膜色素上皮细胞增殖及其作用机制。
Proliferation of retinal pigment epithelial cells induced by (R,R)-XY-10 and (S,S)-XY-10 and their action mechanisms.
作者信息
Cheng Yu-Wen, Wang Yu-Liang, Zhang Yi-Hua, Peng Si-Xun, Chiou George C Y
机构信息
Institute of Ocular Pharmacology, College of Medicine, Texas A&M Health Science Center, College Station, TX 77843, USA.
出版信息
Int J Ophthalmol. 2010;3(1):9-13. doi: 10.3980/j.issn.2222-3959.2010.01.03. Epub 2010 Mar 18.
AIM
To investigate the mechanism of proliferation effect induced by (R,R)-XY-10 and (S,S)-XY-10 on retinal pigmented epithelial cells (ARPE-19).
METHODS
Human retinal pigmented epithelial cells (ARPE-19) and human umbilical vein endothelial cells (HUVECs) were used to investigate the effect of (R,R)-XY-10 and (S,S)-XY-10 on cell growth, and their mechanisms of proliferative action by using ERK, AKT, PI3K, Protein kinase C (PKC) and Nitric oxide synthase (NOS) inhibitors.
RESULTS
(R,R)-XY-10 and (S,S)-XY-10 dose-dependently increased ARPE-19 cell proliferation, but not on HUVECs. When treated with proliferative inhibitors, H-7 (5µmol/L), hypericin (20µmol/L), PD98059 (2µmol/L), LY294002 (50µmol/L), SH-5 (10µmol/L) and L-NAME (100µmol/L), the proliferative effect was reduced by H-7, hypericin, PD98059 and LY294002, but not by SH-5 and L-NAME.
CONCLUSION
(R,R)-XY-10 and (S,S)-XY-10 can induce cell proliferation through MAPK and PI3K dependent pathway.
目的
研究(R,R)-XY-10和(S,S)-XY-10对视网膜色素上皮细胞（ARPE-19）增殖作用的机制。
方法
采用人视网膜色素上皮细胞（ARPE-19）和人脐静脉内皮细胞（HUVECs），研究(R,R)-XY-10和(S,S)-XY-10对细胞生长的影响，以及使用ERK、AKT、PI3K、蛋白激酶C（PKC）和一氧化氮合酶（NOS）抑制剂探讨其增殖作用机制。
结果
(R,R)-XY-10和(S,S)-XY-10能剂量依赖性地增加ARPE-19细胞增殖，但对HUVECs无此作用。当用增殖抑制剂H-7（5μmol/L）、金丝桃素（20μmol/L）、PD98059（2μmol/L）、LY294002（50μmol/L）、SH-5（10μmol/L）和L-NAME（100μmol/L）处理时，H-7、金丝桃素、PD98059和LY294002可降低增殖作用，但SH-5和L-NAME无此作用。
结论
(R,R)-XY-10和(S,S)-XY-10可通过MAPK和PI3K依赖性途径诱导细胞增殖。
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