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香叶基香叶基丙酮对慢性眼压升高大鼠视网膜中热休克蛋白70表达的影响。

Influence of geranylgeranylacetone on the expression of HSP70 in retina of rats with chronic IOP elevation.

作者信息

Liu Zhi-Li, Wang Ying-Rong, Sha Qian, Nie Qing-Zhu

机构信息

Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China.

出版信息

Int J Ophthalmol. 2010;3(1):28-31. doi: 10.3980/j.issn.2222-3959.2010.01.07. Epub 2010 Mar 18.

DOI:10.3980/j.issn.2222-3959.2010.01.07
PMID:22553512
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340655/
Abstract

AIM

To study the effects of geranylgeranylacetone (GGA) on the expression of heat shock protein70 (HSP70) on retinal ganglion cells (RGC) in rats with chronic intraocular pressure (IOP) elevation.

METHODS

Seventy Wistars were divided into blank control group (10 rats), chronic hypertension group (30 rats) and GGA group (30 rats). Chronic hypertension was created by cauterizing the superficial scleral veins. 800mg/kg/d GGA was given by oral daily after cauterization. Immunohistochemistry was used respectively to observe the changes of expression of HSP70 in the model rats and GGA interference rats at different time points during the course of chronic IOP elevation.

RESULTS

The successful model was identified as the IOP over 40% of normal rats. The retinal thickness was significantly reduced in model group and model+GGA group compared with normal rats from 21 days through 28 days after cauterization (P<0.05), and that of model rats was obviously decreased in comparison with model+GGA rats (P<0.05). The number of ganglion cells was significantly decreased in model rats and model+GGA rats compared with normal rats from 21 days and 28 days. The stronger expression intensity (IOD) value was seen for HSP70 in the model+GGA rats by immunochemistry (P<0.01).

CONCLUSION

Systemic administration of GGA protects retina from chronic IOP elevation by regulating the expression of HSP70.

摘要

目的

研究香叶基香叶基丙酮（GGA）对慢性眼压升高大鼠视网膜神经节细胞（RGC）热休克蛋白70（HSP70）表达的影响。

方法

将70只Wistar大鼠分为空白对照组（10只）、慢性高眼压组（30只）和GGA组（30只）。通过烧灼表层巩膜静脉建立慢性高眼压模型。烧灼后每日经口给予800mg/kg/d GGA。分别采用免疫组织化学法观察慢性眼压升高过程中不同时间点模型大鼠和GGA干预大鼠HSP70表达的变化。

结果

成功建立模型的标准为眼压超过正常大鼠的40%。烧灼后21天至28天，模型组和模型+GGA组的视网膜厚度与正常大鼠相比显著降低（P<0.05），且模型大鼠的视网膜厚度明显低于模型+GGA大鼠（P<0.05）。与正常大鼠相比，模型大鼠和模型+GGA大鼠在21天和28天时神经节细胞数量显著减少。免疫化学检测显示，模型+GGA大鼠中HSP70的表达强度（IOD）值更高（P<0.01）。

结论

全身给予GGA可通过调节HSP70的表达保护视网膜免受慢性眼压升高的影响。

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Differential neuroprotection from human heat shock protein 70 overexpression in in vitro and in vivo models of ischemia and ischemia-like conditions.在体外和体内缺血及类缺血状态模型中，人热休克蛋白70过表达产生的差异性神经保护作用。
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