Nie Qing-Zhu, Liu Zhi-Li, Sha Qian, Gao Dian-Wen
Department of Ophthalmology, Shengjing Hospital Affiliated to China Medical University, Shenyang 110004, Liaoning Province, China.
Int J Ophthalmol. 2010;3(2):112-3. doi: 10.3980/j.issn.2222-3959.2010.02.04. Epub 2010 Jun 18.
To study the expressive variation of Nogo-A on rat retina in the process of chronic ocular hypertension.
Thirty-six healthy adult male Wistars were randomly divided into control group (6 rats) and chronic hypertension group (30 rats). Chronic hypertension was created by cauterizing the superficial scleral veins. Immunohistochemistry technique was used to evaluate the expressive varieties of Nogo-A at different time points during the course of chronic ocular hypertension.
The success of the model was indicated by over 40% of increase in the IOP as compared with normal rats. Compared with control group, as time passed chronic hypertension group gradually had detectable morphology changes in the retina. At the 21st day of chronic ocular hypertension, retinas became thinner and the quantity of retinal ganglion cells (RGC) decreased (P<0.05). Assoicated with the morphological changes, the expression of Nogo-A was strongly increased (P<0.05).
Myelin associated protein Nogo-A plays a part in the process of chronic ocular hypertension.
研究慢性高眼压过程中Nogo - A在大鼠视网膜中的表达变化。
将36只健康成年雄性Wistar大鼠随机分为对照组（6只大鼠）和慢性高眼压组（30只大鼠）。通过烧灼表层巩膜静脉创建慢性高眼压模型。采用免疫组织化学技术评估慢性高眼压过程中不同时间点Nogo - A的表达变化。
与正常大鼠相比，眼压升高超过40%表明模型构建成功。与对照组相比，随着时间推移，慢性高眼压组视网膜逐渐出现可检测到的形态学变化。在慢性高眼压第21天，视网膜变薄，视网膜神经节细胞数量减少（P<0.05）。与形态学变化相关，Nogo - A的表达显著增加（P<0.05）。
髓磷脂相关蛋白Nogo - A在慢性高眼压过程中起作用。
