Majima K, Kojima Y, Ouhashi F
Department of Ophthalmology, Fujita Health University, School of Medicine, Aichi, Toyoake, Japan.
Ophthalmologica. 1998;212(6):364-8. doi: 10.1159/000027369.
It has been assumed that lens epithelial cells (LECs) existing at the capsulotomy edge have been traumatized through anterior capsulotomy in cataract extraction. In this study, the correlation between traumatized LECs remaining at the anterior capsulotomy edge and epidermal growth factor (EGF) found in the aqueous humor, a cell growth factor though to affect cell morphology, was determined. Anterior lens capsules with adhering LECs were obtained following anterior capsulotomy performed during cataract surgery to first confirm the presence of EGF receptors on LECs, which are needed for EGF to be biologically active. Besides, to identify any EGF receptors on traumatized LECs, I next intentionally traumatized the cells by pressing them with a forceps from the anterior capsular side. It has been found that the LECs containing EGF receptors were always those existing at the edge of the anterior capsular opening and LECs containing EGF receptors existed along the pressed region too. The present results indicate that traumatized LECs along the capsulotomy edge have undergone changes to manifest EGF receptors, thus allowing EGF from the aqueous humor to become more active. The physiological effect of EGF upon these LECs may therefore be one of the causative factors of fibrous opacification of the anterior capsulotomy edge after cataract extraction.
一般认为，在白内障摘除术中，存在于囊膜切开边缘的晶状体上皮细胞（LECs）因前囊膜切开术而受到损伤。在本研究中，确定了残留在前囊膜切开边缘的受损LECs与房水中发现的表皮生长因子（EGF）之间的相关性，EGF是一种被认为会影响细胞形态的细胞生长因子。在白内障手术期间进行前囊膜切开术后，获取带有附着LECs的前晶状体囊膜，以首先确认LECs上存在EGF受体，这是EGF具有生物活性所必需的。此外，为了识别受损LECs上的任何EGF受体，我接下来从前囊膜侧用镊子挤压细胞，故意损伤这些细胞。已发现含有EGF受体的LECs总是存在于前囊膜开口边缘的细胞，并且含有EGF受体的LECs也存在于挤压区域。目前的结果表明，沿囊膜切开边缘的受损LECs发生了变化，表现出EGF受体，从而使房水中的EGF变得更具活性。因此，EGF对这些LECs的生理作用可能是白内障摘除术后前囊膜切开边缘纤维性混浊的致病因素之一。
