氦氖激光对兔小梁切除术后滤过通道瘢痕形成的影响及机制
Influence and mechanism of He-Ne laser on scar formation of filtration canal after trabeculectomy in rabbit.
作者信息
Wang Rui-Lin, Li Gui-Gang, Chen Zhi-Qi, Xiang Yan, Zhang Hong
机构信息
Department of Ophthalmology, the Affialted Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
出版信息
Int J Ophthalmol. 2010;3(3):192-5. doi: 10.3980/j.issn.2222-3959.2010.03.02. Epub 2010 Sep 18.
AIM
To investigate the influence of He-Ne laser on connective tissue growth factor (CTGF) expression and collagen formation of fibroblast in filtration site after trabeculectomy in rabbit, and to discuss the mechanism for preventing scar formation with He-Ne laser in vivo.
METHODS
The upper nasal limbus area next to the upper rectus muscle in right eyes received 10 minutes He-Ne laser irradiation (200mW/cm(2)) every day for three days, the left eyes served as control. Twenty-four hours after the last irradiation, both eyes of the rabbits were took trabeculectomy surgery. The expressions of CTGF in the filtration area were tested on the 7(th), 14(th) and 28(th) day after surgery and collagen density was tested on the 14(th) and 28(th) day after surgery. Each of the time point had 7 rabbits.
RESULTS
The expression of CTGF was lower than that of the control group's on the 7(th) and 14(th) day after trabeculectomy surgery (P=0.01, P=0.005). When examined on the 14(th) and 28(th) day, the collagen density of irradiation group were significantly lower than that of the control group's (P=0.013, P=0.01).
CONCLUSION
Pretreating the filtration area with 200mW/cm(2) He-Ne laser may be helpful in preventing scar formation after trabeculectomy in rabbit, possibly due to downregulation of the expression of CTGF and collagen synthesis in fibroblasts. He-Ne laser may be developed into a new scar preventing method in filtration surgery.
目的
探讨氦氖激光对兔小梁切除术后滤过部位成纤维细胞结缔组织生长因子（CTGF）表达及胶原形成的影响，探讨氦氖激光在体内预防瘢痕形成的机制。
方法
右眼上直肌旁鼻上缘区每天接受10分钟氦氖激光照射（200mW/cm²），连续照射3天，左眼作为对照。末次照射后24小时，对兔双眼行小梁切除术。术后第7、14和28天检测滤过区CTGF的表达，术后第14和28天检测胶原密度。每个时间点有7只兔。
结果
小梁切除术后第7和14天，CTGF表达低于对照组（P = 0.01，P = 0.005）。在第14和28天检查时，照射组的胶原密度明显低于对照组（P = 0.013，P = 0.01）。
结论
用200mW/cm²氦氖激光预处理滤过区可能有助于预防兔小梁切除术后瘢痕形成，可能是由于成纤维细胞中CTGF表达下调和胶原合成减少。氦氖激光可能发展成为滤过手术中一种新的预防瘢痕形成的方法。
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