Hu Jun, Li Gui-Gang, Zhang Hong, Xiang Nan, Chen Zhi-Qi, Wang Rui-Lin, Xiang Yan, Li Bin, Li Zheng-Jia, He Yan-Yan, Wang Li-Fang
Department of Ophthalmology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
Int J Ophthalmol. 2010;3(2):132-6. doi: 10.3980/j.issn.2222-3959.2010.02.09. Epub 2010 Jun 18.
To investigate the influence of He-Ne lasers on scar formation in the filtration canal after trabeculectomy in a rabbit model, as well as to explore the mechanisms for preventing scar formation when using He-Ne lasers in vivo.
Experiment 1: Four groups were established (four eyes in each group). In 12 eyes, the upper nasal limbus area next to the upper rectus muscle received 10 minutes of He-Ne laser irradiation (100, 150, 200mW/cm(2); 60, 90, 120J/cm(2)) every day for three days. Four eyes served as controls. Twenty-four hours after the final irradiation, the rabbits were sacrificed and the irradiated tissue was excised, fixed with paraformaldehyde and tested for proliferating cell nuclear antigen (PCNA), connective tissue growth factor (CTGF) and apoptosis (TUNEL). Experiment 2: Forty-two rabbits were randomly divided into two groups and standard trabeculectomy was performed in the right eyes either after 200mW/cm(2) He-Ne laser irradiation or not in the filtration area. The expression of PCNA and CTGF, apoptosis and collagen density in the filtration area were tested on the 7(th), 14(th) and 28(th) day after surgery.
Experiment 1: There were no more PCNA and CTGF positive cells in the He-Ne irradiation group than in the control group. No apoptotic cells were found in either group. Experiment 2: The expression of PCNA and CTGF was lower in the He-Ne irradiation group than in the control group on the 7(th) and 14(th) day after trabeculectomy surgery (P<0.05); no apoptotic cells were detected in either group. Collagen density was significantly lower in the He-Ne irradiation group than in the control group on the 14(th) and 28(th) day after surgery (P<0.05).
Pretreating the filtration area with 200mW/cm(2) (120J/cm(2)) of He-Ne laser irradiation may be helpful in preventing scar formation after trabeculectomy, possibly due to the downregulation of the expression of PCNA, CTGF and collagen synthesis in fibroblasts.
研究氦氖激光对兔小梁切除术后滤过通道瘢痕形成的影响，并探讨其在体内预防瘢痕形成的机制。
实验1：设立四组（每组4只眼）。12只眼中，上直肌旁的鼻上象限角膜缘区域每天接受10分钟氦氖激光照射（100、150、200mW/cm²；60、90、120J/cm²），共照射三天。4只眼作为对照。末次照射24小时后处死兔子，切除照射组织，用多聚甲醛固定，检测增殖细胞核抗原（PCNA）、结缔组织生长因子（CTGF）及凋亡情况（TUNEL法）。实验2：42只兔子随机分为两组，右眼在滤过区接受200mW/cm²氦氖激光照射或不照射后行标准小梁切除术。术后第7、14和28天检测滤过区PCNA和CTGF的表达、凋亡情况及胶原密度。
实验1：氦氖激光照射组PCNA和CTGF阳性细胞数不高于对照组。两组均未发现凋亡细胞。实验2：小梁切除术后第7和14天，氦氖激光照射组PCNA和CTGF的表达低于对照组（P<0.05）；两组均未检测到凋亡细胞。术后第14和28天，氦氖激光照射组胶原密度显著低于对照组（P<0.05）。
用200mW/cm²（120J/cm²）氦氖激光照射滤过区可能有助于预防小梁切除术后瘢痕形成，可能是由于成纤维细胞中PCNA、CTGF表达下调及胶原合成减少。
