用眼镜片诱导豚鼠近视模型中5-羟色胺水平及5-HT2A受体mRNA表达
5-hydroxytryptamine level and 5-HT2A receptor mRNA expression in the guinea pigs eyes with spectacle lens-induced myopia.
作者信息
Yang Ji-Wen, Xu Yan-Chun, Sun Lin, Tian Xiao-Dan
机构信息
Department of Ophthalmology, the First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning Province, China.
出版信息
Int J Ophthalmol. 2010;3(4):299-303. doi: 10.3980/j.issn.2222-3959.2010.04.05. Epub 2010 Dec 18.
Abstract
AIM
To investigate 5-hydroxytryptamine (5-HT) function and 5-HT receptor 2A (5-HT2A) mRNA expression in the formation of lens-induced myopia (LIM).
METHODS
Lens-induced myopia construction method was applied to generate myopia on guinea pig right eye (LIM eye).
RESULTS
LIM eyes formed significant myopia with longer axial length. 5-HT level in retina, choroids and sclera from LIM eyes was significantly higher than that in control group. 5-HT2A mRNA expression was also significantly up-regulated.
CONCLUSION
Refraction lens could induce myopia in guinea pig and 5-HT may play an important role in the formation of myopia by binding with 5-HT2A receptor.
摘要
目的
研究5-羟色胺（5-HT）功能及5-羟色胺受体2A（5-HT2A）mRNA表达在晶状体诱导性近视（LIM）形成中的作用。
方法
采用晶状体诱导性近视造模方法在豚鼠右眼（LIM眼）诱导形成近视。
结果
LIM眼形成明显近视且眼轴变长。LIM眼视网膜、脉络膜和巩膜中的5-HT水平显著高于对照组。5-HT2A mRNA表达也显著上调。
结论
屈光性晶状体可诱导豚鼠近视，5-HT可能通过与5-HT2A受体结合在近视形成中发挥重要作用。
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