兔光动力治疗后角膜新生血管的超微结构病理学
Ultrastructural pathology of corneal neovascularization after photodynamic therapy in rabbits.
作者信息
Chen Hong, Zhang Ming-Chang, Wang Zhi, Zhang Ying
机构信息
Department of Ophthalmology, Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, Hubei Province, China.
出版信息
Int J Ophthalmol. 2010;3(4):308-10. doi: 10.3980/j.issn.2222-3959.2010.04.07. Epub 2010 Dec 18.
AIM
To investigate the ultrastructural pathogenesis of photodynamic therapy (PDT) for the experimental corneal neovascularization (CNV) by Hematoporphyrin Derivate (HPD) as photosensitizer and Argon laser as light source.
METHODS
Experimental CNV models were induced in 7 white rabbits using alkali burn. Six weeks after models establishment, animals with CNV were injected with HPD intravenously, and 48 hours after the injection, 7 eyes were irradiated with argon laser (power 800mw, wavelength 514.5nm, spot diameter 200µm, exposure time 2ms). The irradiated CNV was observed by light microscopy and scanning electron microscopy.
RESULTS
Histopathological study indicated that there was a striking decrease in the number of the CNV, vascular endothelium became degeneration and necrosis, some vessels were atrophy and attenuated, and vessels cavity were blocked by some thrombosis. No obvious abnormal histopathological findings were noted in surrounding tissues.
CONCLUSION
The high precise action on CNV and minimal damage to surrounding tissues with PDT by HPD as photosensitizer suggested that PDT might be an effective and safe modality in the treatment of CNV.
目的
以血卟啉衍生物（HPD）为光敏剂、氩离子激光为光源，研究光动力疗法（PDT）治疗实验性角膜新生血管（CNV）的超微结构发病机制。
方法
用碱烧伤法在7只白兔中诱导建立实验性CNV模型。模型建立6周后，给患有CNV的动物静脉注射HPD，注射后48小时，用氩离子激光（功率800mw，波长514.5nm，光斑直径200µm，曝光时间2ms）照射7只眼的CNV。通过光学显微镜和扫描电子显微镜观察照射后的CNV。
结果
组织病理学研究表明，CNV数量显著减少，血管内皮细胞变性坏死，部分血管萎缩变细，血管腔被一些血栓阻塞。周围组织未发现明显的异常组织病理学表现。
结论
以HPD为光敏剂的PDT对CNV具有高精度作用且对周围组织损伤最小，提示PDT可能是治疗CNV的一种有效且安全的方法。
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