COX-2抑制剂NS-398对大鼠真菌性角膜炎中白细胞介素-10表达的影响

Effects of COX-2 inhibitor NS-398 on IL-10 expression in rat fungal keratitis.

作者信息

Li Na, Che Cheng-Ye, Hu Li-Ting, Lin Jing, Wang Qing, Zhao Gui-Qiu

机构信息

Department of Ophthalmology, the Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China.

出版信息

Int J Ophthalmol. 2011;4(2):165-9. doi: 10.3980/j.issn.2222-3959.2011.02.11. Epub 2011 Apr 18.

DOI:10.3980/j.issn.2222-3959.2011.02.11

PMID:22553634

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3340712/

Abstract

AIM

To investigate the expression of interleukin-10 (IL-10) and the effect of NS-398(COX-2 inhibitor) on the expression of IL-10 in fungal keratitis in rats, and analyze its effects on anti-fungus immunity.

METHODS

Ninety Wister rats were randomly divided into 3 groups. Group A was blank control group (10 eyes). Group B was fungal keratitis group (40 eyes). Group C was fungal keratitis group treated with NS-398 (40 eyes). PAS staining, 100g/L potassium hydroxide (KOH) smear and fungal culture confirmed the successful establishment of fungal keratitis model. After the central epithelium was scraped, Fusarium solani colonies were applied and contact lens was put on the right cornea of group B and C, and plane contact lens was put on the left cornea of control eyes. Phosphate buffered saline (PBS) eyedrops were given for group B and NS-398 eyedrops for group C. The expression of IL-10 on corneas of group B and C on the 1(st) day, 3(rd) days, 7(th) days, and 14(th) days were detected by immunohistochemistry and semi- quantitative reverse transcription- polymerase chain reaction (RT-PCR).

RESULTS

Histopathologic examination showed neutrophil infiltration and severe tissue necrosis in ulcer cornea. PAS staining confirmed the existence of hyphae and spores in the superficial layer of stroma. In the blank and control groups almost no expression of IL-10 was detected at any observing points. In group B the expression of IL-10 increased at first and decreased thereafter. Its expression also showed significant difference at any observing points (P<0.01). Compared with group B, the expression of IL-10 in group C showed no difference on the 1(st) day, decrease on the 3(rd) day, but a significant increase on the 7(th) day and 14(th) day.

CONCLUSION

IL-10 takes part in the occurrence and development of fungal keratitis. NS-398 can upgrade the expression of IL-10 in fungal keratitis in the later period of the ulcer. Meanwhile, pathologic observation showed a slightly corneal opacity. IL-10 may play an important role in the process of cornea anti-damage repair.

摘要

目的

研究白细胞介素-10（IL-10）的表达及NS-398（环氧化酶-2抑制剂）对大鼠真菌性角膜炎中IL-10表达的影响，并分析其对抗真菌免疫的作用。

方法

将90只Wister大鼠随机分为3组。A组为空白对照组（10只眼）。B组为真菌性角膜炎组（40只眼）。C组为用NS-398治疗的真菌性角膜炎组（40只眼）。过碘酸雪夫（PAS）染色、100g/L氢氧化钾（KOH）涂片及真菌培养证实真菌性角膜炎模型建立成功。刮除中央上皮后，将茄病镰刀菌菌落接种于B组和C组大鼠右眼角膜并佩戴隐形眼镜，对照组大鼠左眼佩戴平面隐形眼镜。B组给予磷酸盐缓冲液（PBS）滴眼液，C组给予NS-398滴眼液。采用免疫组织化学和半定量逆转录-聚合酶链反应（RT-PCR）检测B组和C组角膜在第1天、第3天、第7天和第14天IL-10的表达。

结果

组织病理学检查显示溃疡角膜有中性粒细胞浸润和严重组织坏死。PAS染色证实基质浅层有菌丝和孢子存在。在空白对照组和正常对照组的任何观察点几乎均未检测到IL-10表达。B组IL-10表达先升高后降低，在任何观察点其表达也有显著差异（P<0.01）。与B组相比，C组IL-10表达在第1天无差异，第3天降低，但在第7天和第14天显著升高。